Table 1.

Drug susceptibilities of viruses containing mutations associated with drug resistancea

MutationFold change in susceptibility (IC50 for sample virus/IC50 for reference virus)
ZDVd4T3TCddCddIABCDLVNVPEFVSQVRTVIDVNFVAMP
NRTI
 M41L,T215Y11.21.52.01.00.92.6
 M41L,D67N,K70R,T215Y,K219Q54.61.93.00.80.92.8
 M184V0.40.8>1501.40.93.8
 M184I0.30.8>1501.51.23.1
 M41L,D67N,K70R,M184V,T215Y,K219Q4.81.4>1501.81.43.4
 M41L,D67N,K70R,Y181C,T215Y,K219Q7.31.41.10.40.51.0
 K65R0.41.712.52.51.82.6
 L74V1.10.92.61.21.8 NTb
 V75T0.61.93.51.41.2NT
 M41L,A62V,T69SSA,L210W,T215Y>1,00013.321.92.13.016.4
 Q151M7.42.91.42.26.8NT
 A62V,V75I,F77L,F116Y,Q151M,M184V307.411.5>15016.118.328.9
NNRTI
 L100I30.03.110.0
 K101E4.911.65
 K103N52.055.026.0
 V106A5.064.01.7
 V108I1.331.7
 Y181C35.0161.03.0
 Y188L9.0>500109.0
 G190A0.575.07.6
 G190S0.4206.047.0
 K101E,G190A2.0>500123.0
 K101E,G190S1.4>500>500
 K103N,Y181C>250>50031
 K103N,G190A37.0>500213.0
PRI
 D30N,N88D0.50.40.57.10.4
 D30N,M46I,L63P,N88D0.91.01.224.70.8
 M46I,L63P,V82T,I84V1.016.12.82.23.0
 L10R,M46I,L63P,V82T,I84V1.722.43.93.64.4
 L10I,L63P,A71V,I84V,L90M9.012.03.66.23.6
 L10I,M46I,L63P,A71V,I84V,L90M4.911.34.211.54.5
 G48V,L90M12.94.43.73.02.3
  • a Resistance test vectors containing defined amino acid substitutions were generated either by site-directed mutagenesis (56) or by isolating RTV clones derived from patient viruses. PR and RT amino acid sequences were verified by nucleic acid sequence analyses.

  • b NT, not tested.