Table 2.

Results of IEF, genotyping of β-lactamases, plasmid analysis, and PFGE

Resistance phenotype group and strainClinical isolateTransconjugantc
pI(s)SHVTEMAmpCPlasmid profilePFGE patternpISHVAmpCApprox plasmid size (kb)
Group I
 X3868.2, 7.65AI8.25100
 C0978.2, 7.6, 5.451BII8.25100
 Q3818.2, 7.6, 5.451CIII8.2570
 T923a 8.2, 7.6, 5.451DIV
 T3868.2, 7.6, 5.4121EV8.21270
 R4368.2, 7.6, 5.4121EVI8.212100
 S2768.2, 7.6, 5.4121FVII8.212100
 T9868.2, 7.6, 5.4121GVIII8.21270
 B262a 8.2, 7.6, 5.4121HIX
 T255a 8.2, 7.6, 5.4121IX
 V2938.2, 7.6, 5.4121GXI8.21270
 D1558.2, 7.612JXII8.21240
 B6578.2, 7.612KXIII8.21275
 C1068.2, 7.612JXIV8.21240
Group II
 O5748.3, 7.6, 5.4111LXV8.395
 R5498.3, 7.6, 5.4111MXVI8.3125
Group III
 W1428.25, 7.6, 5.4111CMY-8NXVII8.25CMY-825
 W5808.25, 7.6, 5.4111CMY-8NXVII8.25CMY-825
 T3848.25, 7.6, 5.4111CMY-8NXVII8.25CMY-825
Group IV
 O7878.2, 7.6, 5.4121UDb OXVIII8.212UD150
  • a Conjugation experiments with strains T923, B262, and T255 were not successful.

  • b UD, undetermined.

  • c All transconjugates lacked TEM.