Table 5.

Genotypic characterization of actinonin-resistantS. aureus mutantsa

S. aureus strainfmt genotypeMIC (μg/ml) of actinonin in the presence of:
No plasmidpPV158-1
ATCC 25923Wild type16 NDb
VSAU6011A108E>1284
VSAU6012G117V>128ND
VSAU6013E157Stop>128ND
1-63Wild type164
VSAU6014Frameshiftc >1288
RN4220Wild type162
VSAU7136Deletion>1284
  • a Values for uncomplemented strains are repeated from Table 4. For strains that harbored pPV158-1 (which supplies fmt in trans), MICs were determined in the presence of 10 μg of tetracycline per ml.

  • b ND, not determined.

  • c A frameshift at codon 180 results in translation termination at codon 184.