Table 1.

Nucleotide sequences of the oligonucleotides used for amplification and/or sequencing reactions

PrimeraSequencecPosition 5′b
Amplification and/or sequencing
 A5′-TAAAATTCTTGAAGACG-3′−5
 C5′-GGGCAAGAGCAACTCGG-3′461
 F5′-TTACTGTCATGCCATCCG-3′560
 P35′-CTGACTCCCCGTCGTGT-3′1003
 B5′-TTACCAATGCTTAATCA-3′1069
ASPCR
 P15′-GGGCCTCGTGATACGCC-3′16
 P25′-----------------T-3′
 Gln 395′-AAAGATGCTGAAGATC-3′302
 Lys 395′----------------A-3′
 Glu 1045′-CTCAGAATGACTTGGTTG-3′495
 Lys 1045′------------------A-3′
 Gly 2385′-GCGAGACCCACGCTCACC-3′931
 Ser 2385′------------------T-3′
 Arg 2445′-TGCTGCAATGATACCGCG-3′946
 Ser 2445′------------------T-3′
 Cys 2445′------------------A-3′
  • a Ambler et al. numbering (1).

  • b Sutcliffe numbering (35).

  • c A dash indicates that the nucleotide is the same as in the sequence above.