Table 3.

Membrane sterol levels in K. lactis andK. lactis mutant strains grown in the presence or absence of AmBa

CompoundRelative retention timeb% of sterol content in strain
K. lactisK. lactis + AmBK. lactis mutantK. lactis mutant + AmB
Cholesterol11 ± 0.23 ± 0.45 ± 0.46 ± 0.5
Dehydroergosterol1.082 ± 0.35 ± 0.52 ± 0.48 ± 0.7
Zymosterol1.123 ± 0.612 ± 1.09 ± 0.813 ± 1.1
Dihydroergosterol1.205 ± 0.517 ± 1.53 ± 0.45 ± 0.6
Ergosterol1.2381 ± 437 ± 465 ± 344 ± 5
4-Methylzymosterol1.272 ± 0.4tr12 ± 0.97 ± 0.7
Fecosterol1.28tr3 ± 0.3tr2 ± 0.3
Stigmasterol1.35tr2 ± 0.3trtr
Episterol1.372 ± 0.25 ± 0.31 ± 0.17 ± 0.6
Ergosta-7-ene-3-ol1.40trtrtr2 ± 0.2
Lanosterol1.452 ± 0.39 ± 1.31 ± 0.25 ± 0.5
4,4-Dimethylzymosterol1.51tr6 ± 0.51 ± 0.2tr
4,4-Dimethyl-ergosta-8,24(28)diene-3-ol1.731 ± 0.2trtrtr
  • a AmB was used at subinhibitory doses (10 and 125 mg/liter for K. lactis and the K. lactismutant, respectively).

  • b Retention times are expressed as ratio to cholesterol retention time (1).