Table 1.

Effect of the C-8-methoxy fluoroquinolone PD161148 against clinical isolates of M. tuberculosis

Isolate no.MIC (μg/ml)MPC (μg/ml)aIS6110 typebGenotype groupcSourcedAntibiotic resistancee
TN16260.11.0W1N.Y.I, S, R, Em, K
TN78110.11.0Unique3RussiaS, K
TN78040.11.0W1481RussiaI, S, R, Et, K
TN65150.071.5W41N.J.None
TN77910.11.5Unique2RussiaI
TN78290.12.3Unique3RussiaNone
TN78340.12.3Unique3RussiaNone
TN57040.22.3Unique1N.J.I
TN53550.22.3Unique3N.J.None
TN54100.22.3Unique2N.J.None
TN35410.22.3DV1N.Y.I, R, Em
TN18650.33.0f H2N.Y.None
TN21560.33.5CO2N.Y.None
TN57020.44.0f Unique1N.J.I
  • a The MPC was determined as the PD161148 concentration required to prevent the recovery of resistant mutants when >2 × 109 CFU was applied to each of five agar plates. Each experiment was performed twice, with results similar to those shown obtained each time.

  • b IS6110 DNA pattern as described previously (11).

  • c Genotype group as described previously (16).

  • d From the Public Health Research Institute Tuberculosis Center collection. Abbreviations: N.Y., New York; N.J., New Jersey.

  • e Isolates were resistant to the indicated antibiotics: I, isoniazid; S, streptomycin; R, rifampin; Em, ethambutol; K, kanamycin; Et, ethionamide.

  • f Determined by extrapolation from linear plots as in inset to Fig. 2.