Table 1.

Effects of substances possibly interfering with the hematin polymerization reaction

Class and compoundConcn% InhibitionIC50Concn tolerated
Cations and anions
 NaCl100 mM10
 KCl100 mM10
 NH4Cl100 mM14
 MgCl2100 mM16
 CaCl2100 mM−15
 Na2HPO4100 mM26
 NaH2PO4100 mM14
Detergents
 Tween 801%880.14%0.003%
 Triton X-1001%37
 SDS1%46
Chelating agents
 EDTA10 mM−19
 EGTA10 mM−10
Tannins
 Catechin100 μg/ml11
 Gallic acid100 μg/ml−43
 Tannic acid100 μg/ml10034 μg/ml3 μg/ml
Pigments
 Chlorophyll a100 μg/ml33
 Chlorophyll b100 μg/ml28
 Melanin100 μg/ml40
Saponin (saikosaponin A)100 μg/ml−9
Antibiotics
 Daunomycin100 μg/ml−27
 Amphotericin B100 μg/ml4
 Penicillic acid100 μg/ml−19
 Actinomycin D100 μg/ml37
SH-group reacting agents
 DTT100 μg/ml895.1 μg/ml1 μg/ml
 Cysteine100 μg/ml−13
 GSH100 μg/ml41
Fatty acids
 Stearic acid100 μg/ml11
 Oleic acid100 μg/ml30
 Linoleic acid100 μg/ml−15
Proteases
 Actinase E10 μg/ml−13
 S peptidase10 μg/ml−29
 Proteinase K10 μg/ml−6
Organic solvents
 MeOH10%−29
 EtOH10%−28
 Acetonitrile10%−40
 DMSO10%−37