Table 1.

Bacterial strains

StrainRelevant propertiesReference or source
S. marcescens clincal isolates
 S61982 Imipenem-resistant strain from London, producing SME-1 12
 41761985 Imipenem-resistant strain from Minnesota (MN-2701)Medeiros and Hare, 26th ICAAC
 41261992 Imipenem-resistant strain from UCLA (UCLA-1)Quinn et al., 37th ICAAC
 41241995 Imipenem-resistant strain from Boston (no. 4)Carmeli et al., 37th ICAAC
E. coli transformants
 4911Carries plasmid pIRS1, SME from 4176 cloned into pCR2.1This work
 4912Carries plasmid pIRS2, SME from 4126 cloned into pCR2.1This work
 4913Carries plasmid pIRS3, SME from S6 cloned into pCR2.1This work
 4914Carries plasmid pIRS4, SME from 4124 cloned into pCR2.1This work
E. coliOne ShotRecipient for sme genes cloned into pCR2.1Invitrogen
E. coli One Shot/pCR2.1Vector-only control for MIC testingInvitrogen