Table 1.

Muropeptide composition of peptidoglycan from serotype Typhimurium strain SL1344 carrying pBGS18and derivatives

SamplebRelative abundance (mol%)aCross-linkage (%)c
MonomersDimersTrimersLppAnhydrous forms
d-dl-d
SL1344(pBGS18)65.5 ± 0.622.7 ± 0.69.0 ± 0.52.8 ± 0.412.1 ± 0.63.9 ± 0.337.2 ± 0.3
SL1344(pBGTEM-24)63.4 ± 0.323.4 ± 0.39.9 ± 0.23.3 ± 0.213.1 ± 0.39.1 ± 0.439.9 ± 0.4
SL1344(pBGMHN1)68.1 ± 0.522.6 ± 0.47.3 ± 0.31.9 ± 0.38.3 ± 0.64.9 ± 0.533.8 ± 0.5.
  • a Muropeptides are grouped according to structural similarities as described previously (14). Values are means and standard deviations from three independent experiments.d-d, cross-linked by ad-d peptide bridge;l-d, cross-linked by anl-d peptide bridge; Lpp, muropeptides bound to the C-terminal dipeptide (Arg-Lys) of Braun's lipoprotein.

  • b Peptidoglycan of serotype Typhimurium SL1344 transformed with the indicated plasmids.

  • c Proportion of cross-linked peptide chains.