Table 1.

Strains and plasmids used in this study

Strain or plasmidGenotypeaSource, reference, or comment
S. aureus
 ISP7948325pig-131 25
 MT58325nov (gyrB142) hisG15 pig-131 10, 26
 SS18325pig-131 nov (gyrB142) gyrA(Ser84Leu) 1
 MT5224c28325nov (gyrB142) hisG15 pig-131 flqA552 21
 MT5224c38325nov (gyrB142) hisG15 pig-131 flqA552 26
 MT1118325 pig-131 grlA548 (Ala116Glu) 26
 MT5224c48325 nov (gyrB142) hisG15 pig-131 grlA542 (Ser80Phe) 26
 MT5224c98325 nov (gyrB142) hisG15 pig-131 grlB543 (Arg470Asp) 10, 26
 MT231428325 pig-131 flqBΩ(chr::Tn916)1108 20
 EN1252a8325 nov (gyrB142) hisG15 pig-131 grlA542 (Ser80Phe) gyrA (Ser84Leu) Ω1051 (Erm) Nov+ 1
 MT12228325 pig-131 grlA553 (Ala116Glu) flqB gyrA (Ser84Leu) 26
 RN42208325-4, r 15
 EN6RN4220nov-142 gyrA 21
 EN14RN4220grlB543 Ω(chr::Tn917lac)2 nov (gyrB142) gyrA(Ser84Leu) 21
 EN20RN4220grlA542Ω(chr::Tn917lac)2 21
 EN9RN4220 grlA543Ω(chr::Tn917lac)2 nov(gyrB142) gyrA 21
 P4ISP794 grlA552 (Arg43Cys)This study; spontaneous PRFX-Rb
 P5ISP794grlA553 (Ala116Glu)This study; spontaneous PRFX-R
 P6ISP794 grlA554 (Pro157Leu)This study; spontaneous PRFX-R
 P10ISP794 grlA555(Ala176Thr)This study; spontaneous PRFX-R
 P21ISP794grlA556 (Asp69Tyr)This study; spontaneous PRFX-R
 DIP4ISP794 grlA552 This study, allelic exchange mutant
 DIP6ISP794 grlA554 This study; allelic exchange mutant
 DIP10ISP794 grlA555 This study; allelic exchange mutant
 DIP21ISP794grlA556 This study; allelic exchange mutant
 ISP21338325 pig-131 trp-489Ω(chr::Tn917lac)2 26
 ISP21348325 pig-131 trp-489Ω(chr::Tn917lac)1 26
 ISP225Ps55 26
E. coli
 DH5αF-φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 phoA hsdR17(rK mK )supE44λ thi-1 gyrA96 relA1 Gibco-BRL
Plasmids
 pGEM3-zf(+)3,199-bp cloning vector, Apr Promega
 pCL52.18,100-bp plasmid containing the replicon of pGB2, Spr (E. coli) and temperature-sensitive replicon of pE194, Tcr 16
  • a Abbreviations: Ap, ampicillin; Sp, spectinomycin; Tc, tetracycline.

  • b PRFX-R, premafloxacin resistance.