Table 1.

rpoB mutations detected in 100 independent cultures

PlateaColony countMutationb
2  1H526R
3  1S531L
4  1S531L
6  2S531L
7  1S531L
9  2S531L
1027S531Lc
11  3H526Y
1211S522L
13  1S531W
14  4S531L
18  1S531L
20  6H526Y
25  1S531L
27  2S531L
28  1H526R
29  2S531L
31  3H526D
33  1Deletion
34  1H526R
35  1S531L
37  1S531L
39  3S522L
41  1S531L
42  1H526D
44  1S531L
45  1S531L
47  4H526P
49  4H526R
50  2S531L
53  3S531L
55  3S531L
57  1D516V
59  2H526Y
60  4S531L
61  5H526R
62  1S531L
64  1S531L
66  2S531L
67  1S531L
68  1S531W
69  2S531L
70  3S522L
71  1H526Y
72  1Insertion
73  1S531L
74  3S531L
76288S531Ld
77  5H526D
78  1S531L
79  3S531L
80  2S531L
82  1S531L
83  2H526D
84  2S531L
85  1H526Y
88  1S531L
89  5S531L
90  9S531L
91  3S531L
93  5S522L
96  2S531L
98  2H526Y
100  1S531L
  • a  The other 36 plates yielded no colonies or colonies that failed to grow on subculture in broth.

  • b Corresponding codon changes are listed in Table 2.

  • c Ten colonies were picked for sequencing. The electropherogram for one colony showed a mixture of S531L and H526Y. The other nine colonies gave S531L.

  • d Ten colonies were picked for sequencing; all gave S531L.