Table 6.

Genetic linkage of grlA mutations and resistance by transformation: outcross of mutations

Donor strainaGenotypeMIC (μg/ml) for resistant transformantsb% Resistant (no. of resistant strains/total no. of transformants)
PRFXCPFX
P43355 grlA(Arg43Cys) Ω(chr::Tn917lac)2 0.016–0.0321.019 (23/120)
P6332 grlA (Pro157Leu) gyrA (Met538Ile) Ω(chr::Tn917lac)2 0.016–0.0320.5–1.016 (17/104)
P1033232 grlA (Ala176Thr) Ω(chr::Tn917lac)2 0.016–0.0321.028 (54/192)
P213360 grlA (Asp69Tyr) Ω(chr::Tn917lac)2 0.016–0.0321.020 (14/70)
  • a Recipient ISP794grlA + grlB +

  • b Abbreviations: PRFX, premafloxacin; CPFX, ciprofloxacin.