TABLE 1.

Bacterial strains used in this study

StrainCharacteristic(s)Source or reference
E. coli
    DH5αendA1 hsdR17 (rk mk+) supE44 thi-1 recA gyrA relA1 φ80dlacZΔM15 Δ(lacZYA-argF)Bethesda Research Laboratories
    ATCC 25922Reference strain for MIC testsAmerican Type Culture Collection
    E. coli(pFY13-9BSRX)DH5α with plasmid pFY13-9BSRX carrying the entire ampR1 and blaXCC-1 genes, Kmr/ApsThis study
    E. coli(pBX)DH5α with plasmid pBX carrying the blaXCC-1 promoter situated upstream of the luxA and fluxB reporter genes, Tcr/ApsThis study
    E. coli(pRX)DH5α with plasmid pRX carrying the ampR1 promoter situated upstream of the luxA and luxB reporter genes, Tcr/ApsThis study
X. campestris pv. campestris
    Xc2Wild-type strain isolated in Taiwan, AprTseng lab collectiona
    Xc6Wild-type strain isolated in Taiwan, AprTseng lab collection
    Xc85Wild-type strain isolated in Taiwan, AprTseng lab collection
    Xc17Wild-type strain isolated in Taiwan, AprYang and Tseng (33)
    Xc11Wild-type strain isolated in Taiwan, AprYang and Tseng (33)
    Xc11ASpontaneous avirulent mutant from Xc11, AprYang and Tseng (33)
    Xc11bla::TcXc11 blaXCC-1 mutant constructed by inserting a Tcr cartridge into the SmaI site of the blaXCC-1 gene, Tcr/ApsWeng et al. (29)
    Xc11RMXc11 ampR1 mutant constructed by inserting a Tcr cartridge into the SalI site of the ampR1 gene, Tcr/ApsThis study
    Xc11bla::Tc(pRKSE)Xc11 blaXCC-1 mutant complemented with cloned blaXCC-1 gene, Tcr/Apr/KmrWeng et al. (29)
    Xc11RM(pFJ-ampR1)Xc11 ampR1 mutant complemented with cloned ampR1 gene, Tcr/Apr/KmrThis study
X. campestris pv. begoniae 59Wild-type strain isolated in Taiwan, AprTseng lab collection
X. campestris pv. dieffenbachiae 65Wild-type strain isolated in Taiwan, AprTseng lab collection
X. campestris pv. mangiferaeindcae 38Wild-type strain isolated in Taiwan, ApsTseng lab collection
X. campestris pv. phaseoli 73Wild-type strain isolated in Taiwan, AprTseng lab collection
X. axonopodis pv. citri 60Wild-type strain isolated in Taiwan, AprTseng lab collection
X. axonopodis pv. glycines 69Wild-type strain isolated in Taiwan, AprTseng lab collection
X. oryzae pv. oryzae 21Wild-type strain isolated in Taiwan, AprTseng lab collection
X. axonopodis pv. vesicatoria
    Xv64Wild-type strain isolated in Taiwan, AprTseng lab collection
    Xv3240European strain, ApsOjanen et al. (20)
Serratia marcescens 6Wild-type strain isolated in Taiwan, AprL. T. Wu, China Medical University
  • a Yi-Hsiung Tseng's laboratory collection.