TABLE 2.

Synthetic oligonucleotides primers in mecA genea

Primer purpose and designationSequencebNucleotide positions
Sequencing
    K105′-AGTAGCTCAACGAGCTGAAAAT-3′29943-29964
    K195′-ATACGGAGAAGAAGTTGTAGCA-3′33658-33637
    K155′-AGCACACCTTCATATGACGTCT-3′32473-32494
    K165′-TGGATCAAAATTGGGTACAAGA-3′31991-32012
    K175′-AGTTGTAGTTGTCGGGTTTGGT-3′31425-31446
    K185′-TGGCAATATTAACGCACCTCACT-3′33012-33034
Plasmid construction
    K345′-TATGCGGATCCTCGTGTCAGATACATTTCGATTCA-3′31068-31091
    K385′-ATTTCGGATCCGTTGTAGCAGGAACACAAATGAATAAC-3′33645-33619
    K2375′-CATCTTACAACTAATGAAACAAAAAGTCGTAACTATCCTC-3′32083-32122
    K237rc5′-GAGGATAGTTACGACTTTTTGTTTCATTAGTTGTAAGATG-3′32122-32083
    E4705′-CAAGATAAAGGAATGGCTAACTACAATGCCAAAATCTCAG-3′33298-33337
    E470rc5′-CTGAGATTTTGGCATTGTAGTTAGCCATTCCTTTATCTT-3′33337-33297
  • a The primers are specific for SCCmec around the mecA region of strain NCTC10442 (DDBJ/EMBL/Genbank accession no. AB033763 [12]).

  • b The BamHI sites introduced are underlined.