TABLE 2.

Effects of imipenem on planktonic P. aeruginosa genes

Gene no.aDescriptionFold change in expression level (P value)b
0376rpoH, sigma factor−1.1 (3.3E-1)
0464creC, two-component sensor−1.3 (7.0E-1)
0465creD, inner membrane protein−1.2 (8.4E-1)
0762algU, sigma factor1.2 (5.5E-1)
0763mucA, anti-sigma factor1.1 (7.0E-1)
0764mucB, regulator for alginate biosynthesis1.1 (9.3E-1)
0765mucC, regulator for alginate biosynthesis−1.0 (9.6E-1)
1078flgC, flagellar basal-body rod protein−1.2 (3.7E-1)
1079flgD, flagellar basal-body rod protein−1.3 (3.7E-1)
1080flgE, flagellar hook protein−1.1 (8.6E-1)
1081flgF, flagellar basal-body rod protein−1.1 (5.3E-1)
1084flgI, flagellar P-ring protein precursor−1.2 (4.7E-1)
1178oprH, outer membrane protein H1 precursor1.6 (3.8E-2)
2659Hypothetical protein2.1 (3.3E-2)
3540algD, GDP-mannose 6-dehydrogenase2.9 (1.4E-1)
3541Alginate biosynthesis protein1.9 (2.1E-1)
3542Alginate biosynthesis protein1.5 (1.2E-1)
3543algK, alginate biosynthetic protein4.8 (2.3E-1)
3544algE, outer membrane protein1.7 (2.0E-1)
3545algG, alginate-c5-mannuronan-epimerase1.8 (3.0E-1)
3546algX, alginate biosynthesis protein1.6 (3.3E-1)
3547algL, poly(beta-d-mannuronate) lyase precursor1.4 (2.5E-1)
3548algI, alginate O-acetyltransferase1.2 (6.5E-1)
3549algJ, alginate O-acetyltransferase1.3 (4.3E-1)
3550algF, alginate O-acetyltransferase3.0 (1.7E-1)
3551algA, phosphomannose isomerase/guanosine1.9 (2.7E-1)
4110ampC, β-lactamase precursor19.1 (4.2E-2)
4407ftsZ, cell division protein1.1 (4.6E-1)
4408ftsA, cell division protein1.2 (1.3E-1)
4409ftsQ, cell division protein1.3 (9.4E-2)
4410ddlB, d-alanine-d-alanine ligase1.2 (2.0E-1)
4411murC, UDP-N-acetylmuramate-alanine ligase1.1 (4.7E-1)
4412murG, transferase1.3 (8.6E-2)
4413ftsW, cell division protein1.1 (5.0E-1)
4414murD, UDP-N-acetylmuramate-d-glutamate ligase1.4 (8.4E-2)
4415mraY, phospho-N-acetylmuramate-peptidylpept.-transferase1.2 (1.2E-1)
4416murF, UDP-N-acetylmuramate-d-glutamyl-diaminopimelate-d-alanyl-d-alanyl ligase1.3 (9.9E-2)
4417murE, UDP-N-acetylmuramoylalanyl-d-glutamate-diaminopimelate ligase1.1 (3.6E-1)
4419ftsL, cell division protein1.2 (7.2E-1)
4420Hypothetical protein1.4 (4.4E-1)
4421Hypothetical protein1.2 (5.8E-1)
4525pilA, type 4 fimbrial precursor−1.3 (5.7E-1)
4526pilB, type 4 fimbrial biogenesis protein−1.2 (5.6E-1)
5040pilQ, type 4 fimbrial biogenesis protein−1.3 (2.2E-1)
5041pilP, type 4 fimbrial biogenesis protein−1.2 (2.8E-1)
5042pilO, type 4 fimbrial biogenesis protein−1.2 (2.8E-1)
5044pilM, type 4 fimbrial biogenesis protein−1.1 (5.6E-1)
5261algR, alginate biosynthesis regulatory protein1.3 (4.9E-1)
5262algZ, alginate biosynthesis protein1.3 (5.0E-1)
5322algC, Phosphomannomutase1.1 (1.0E-1)
  • a Gene number from the P. aeruginosa genome project (http://www.pseudomonas.com). Genes indicated in boldface passed the t test (P < 0.05). Arrows indicate the transcriptional orientations of the operons of interest.

  • b E, × 10 (e.g., 3.3E−1 = 3.3 × 10−1).