TABLE 2.

Sequences of the primers used to detect bla genes and their promoter regions

PCR targetPrimer namePrimer sequencea
blaCTX-M (CTX-M-1 group)M13 upper5′-GGT TAA AAA ATC ACT GCG TC-3′
M13 lower5′-TTG GTG ACG ATT TTA GCC GC-3′
blaCTX-M (CTX-M-2 group)M25 upper5′-ATG ATG ACT CAG AGC ATT CG-3′
M25 lower5′-TGG GTT ACG ATT TTC GCC GC-3′
blaCTX-M (CTX-M-9 group)M9 upper5′-ATG GTG ACA AAG AGA GTG CA-3′
M9 lower5′-CCC TTC GGC GAT GAT TCT C-3′
blaTEMOT35′-ATG AGT ATT CAA CAT TTC CG-3′
OT45′-CCA ATG CTT AAT CAG TGA GG-3′
blaCMYCF15′-ATG ATG AAA AAA TCG TTA TGC-3′
CF25′-TTG TAG CTT TTC AAG AAT GCG C-3′
tnpISEcp1bISEcp15′-AAA AAT GAT TGA AAG GTG GT-3′
MA1 reverse5′-ACY TTA CTG GTR CTG CAC AT-3′
tnpIS26IS265′-AGC GGT AAA TCG TGG AGT GA-3′
MA1 reverse5′-ACY TTA CTG GTR CTG CAC AT-3′
IS903IS903 reverse5′-CGG TTG TAA TCT GTT GTC CA-3′
M1 IS9035′-CGT CGG CGG CTA AAA TCG TC-3′
M9 IS9035′-CTA CGG CAC CAC CAA TGA TA-3′
ORF513ORF5135′-TGG AAG AGG GCG AAG ACG AT-3′
M2 reverse upper5′-CGA ATG CTC TGA GTC ATC AT-3′
M9 reverse upper5′-TGC ACT CTC TTT GTC ACC AT-3′
ORF1005ORF1005 reverse5′-ATC CAT AAT AGC ATC CAT CAT-3′
M9 reverse lower5′-GAG AAT CAT CGC CGA AGG G-3′
sul1sul1 reverse5′-GCT CAA GAA AAA TCC CAT CCC C-3′
M2 reverse lower5′-GCG GCG AAA ATC GTA ACC CA-3′
M9 reverse lower5′-GAG AAT CAT CGC CGA AGG G-3′
ORF1M3 int upp5′-TCA CCC AGC CTC AAC CTA AG-3′
ORF1 pol M35′-GCA CCG ACA CCC TCA CAC CT-3′
  • a Y = C or T; R = A or G.

  • b tnp, transposase.