TABLE 3.

Characteristics of ESBL-producing clinical isolates and their transformants, including β-lactam MICs for the clinical strains, their E. coli J53-2 transconjugants, and E. coli DH10B transformants and non β-lactam antibiotic resistance markers

StrainapI by IEFResult by:CTX-M identified by sequencingMIC (μg/ml)bAssociated antibiotic resistance markersc
PCR for blaCTX-MPCR for blaCMYPCR for blaTEMPCR-RFLP analysisCTXCTX-CACAZCAZ-CAFEPFEP-CAATMATM-CAIPM
TN035.4, 7.6M-1/M-3+TEM-1CTX-M-151280.06640.51280.121280.060.12G, T, Nt, Te
Ep TN035.4, 7.6+64323264G, T, Nt, Te
TN058.4M-9CTX-M-9320.120.50.03320.1240.060.12G, T, Nt, Te, Tp, Su
Ep TN058.4320.544G, T, Nt, Tp, Su
TN065.4, 8.2M-2/M-5+TEM-1CTX-M-21280.5640.564112810.12G, T, Nt, Te, C, Tp, Su
Ep TN065.4, 8.2+640.53264G, T, Nt, Su
TN078.4M-9CTX-M-14320.120.50.1240.1280.060.12G, T, Te, C, Tp, Su
Ep TN078.440.544None
TN085.4, 7.6M-1/M-3+TEM-1CTX-M-151280.256421280.12640.250.12T, Nt, A, Te, Tp, Su
Tc TN085.4, 7.6+32321616T, Nt, A, Te, Tp, Su
TN095.4, 7.6M-1/M-3+TEM-1CTX-M-151280.25642640.121280.250.12G, T, Nt, Te
Ep TN095.4, 7.6+1286464128G, T, Nt, Te
TN125.4, 7.6M-1/M-3+TEM-1CTX-M-151280.25641320.5640.50.12G, T, Nt, Te, C, Tp, Su
Tc TN125.4, 7.6+64323264C
TN135.4, 8.4, >9M-9++TEM-1CTX-M-14128321286416132320.25Te, C
Tc TN138.440.120.50.5C
TN145.4, 7.6M-1/M-3+TEM-1CTX-M-151280.56421280.251280.50.12G, T, Nt, Te
Ep TN145.4, 7.6+1286464128G, T, Nt, Te
TN155.4, 7.6M-1/M-3+TEM-1CTX-M-151280.25641320.5640.50.12G, T, Nt, Te, C, Tp, Su
Tc TN155.4, 7.6+32321616G, T, Nt, Te, Tp, Su
TN167.8M-1/M-3CTX-M-1320.2520.2580.12160.120.12Tp, Su
Ep TN167.8322816Tp, Su
TN175.4, 7.6M-1/M-3+TEM-1CTX-M-156416328321664320.12G, T, Nt, Te, C, Tp, Su
Tc TN177.68248G, T, Nt, Te
TN185.4, 7.6M-1/M-3+TEM-1CTX-M-151283264326416128320.12G, T, Nt, Te
Ep TN185.4, 7.6+64326464G, T, Nt, Te
TN198.2M-2/M-5CTX-M-2128416264412840.25Te, Tp, Su
Ep TN198.264166464Te, Tp, Su
RB-017.8M-1/M-3CTX-M-1640.0640.12640.12320.060.25Te, C, Tp, Su
Tc RB-017.840.1242Tp, Su
LM-017.6M-1/M-3CTX-M-15>128464264212820.12T, Nt, A, Te
Tc LM-017.664163232T, Nt, A, Te
KP-LT8M-1/M-3CTX-M-332214160.25320.50.12G, T, Nt, Te, C, Tp, Su
Tc KP-LT84111G, T, Nt, Tp
KP-027.8M-1/M-3CTX-M-140.060.250.2520.1210.030.12Tp, Su
Tc KP-027.84111Tp
KP-035.4, 7.6M-1/M-3+TEM-1CTX-M-15>1284>128321284>12880.25G, T, Nt, A, C, Tp, Su
Ep KP-035.4, 7.6+1286416128T, Nt, A, Tp, Su
  • a Ep, electroporant; Tc, transconjugant.

  • b CTX, cefotaxime; CA, clavulanic acid (2 μg/ml); CAZ, ceftazidime; FEP, cefepime; ATM, aztreonam; IPM, imipenem.

  • c G, gentamicin; T, tobramycin; Nt, netilmicin,; A, amikacin; Te, tetracycline; C, chloramphenicol; Tp, trimethoprim; Su, sulfamides.