TABLE 3.

Genetic analysis of MexAB-OprM- and MexXY-overproducing strains of P. aeruginosa

StrainNucleotide sequencing dataaMean gene expressionb
mexZcmexRdPA3721eVicinity of PA3721mexXmexAPA3720
PAO11.01.01.0
PT629 (nalB)ND4 bp deleted at posi- tion 3630.63.61.71
ATM4 (nalB agrZ) CAG→TAG (C307T) GCA→CCA (A66P)4826.50.11
WL224844, 12671, 153G−73→A, A+13→T1689.22.9
WL2471, 20922.05.10.2
1113 GCC→-CC (A35), ATG→ AT-(M34)12671, 209T−2→C, C inserted (−25)29.64.45.5
1217144, GAG→-AG (E157)7198.56.00.5
123748, CAG→TAG (Q10Z)44, 12671, 153, 209, TCA→GCA (S46A)A+13→T2727.73.7
1250GGT→G--(G46), GCG→- CG (A47)500 bp deleted31217.9147
1562144711298.51.7
17277-bp insertion between 355 and 36171, GAT→GAA (D76E)30034.89.9
173871, 209, CTG→CCG (L61P)82.211.7224
20854844, 12671, 153T−2→C2048.75.8
2151GTC→GCC (V44A)44, 12671, 153, 209T−2→C, A+13→T1102.66.7
2172GGT→AGT (G46S)71, ATG→ACG (M151T)95.46.1194
  • a Nucleotide differences compared with the PAO1 genome (www.pseudomonas.com ).

  • b Relative to PAO1; mean values from two independent experiments.

  • c 48, substitution GTC→GCC (V48→A); 144, GCG→GTG (V144→A).

  • d 44, substitution AAG→ATG (K44→M); 126, GTG→GAG (V126→E).

  • e 71, substitution GGG→GAG (G71→E); 153, GAG→CAG (E153→Q); 209, AGC→CGC (S209→R).