TABLE 5.

Changes in azole susceptibility resulting from expressing C. albicans ERG11 alleles in S. cerevisiae

Original C. albicans isolateSubstitution(s) in expressed C. albicans Erg11pMICa (μg/ml)
POSITZFLZVOR
NoneNone (vector alone)0.060.12540.016
C43None (wild type)0.060.5160.125
C441K143R0.251>2561
C587G464S0.251>2561
C477K128T, G464S, R467I0.1250.5320.25
C530G450E, G464S111281
C600Y132H, S405F0.5>8>25616
C535Y132H, G448V0.251>2560.03
C572Y132H, G450E0.52>2568
C438Y257H, G464S0.511284
C439Y257H, G464S, G307S11>2568
C440Y257H, G464S, G307S, A61V48>25616
NAbA61V0.250.5160.125
  • a See Table 2, footnote a.

  • b NA, not applicable; mutation was engineered into a wild-type ERG11 gene.