TABLE 2.

MICs of antifungal agents in the A. fumigatus strains tested in this studya

StraindMIC (μg/ml)
TRBAMBITCVRC
TRBR>256110.5
rTRBR>256110.5
TRBR-G>256110.5
TRBR-E0.5110.5
TRBR-Lb1110.5
AF293.1b1110.5
QC strainc110.50.25
  • a NCCLS M38-A microdilution method; liquid MM-U medium was used instead of liquid RPMI 1640 medium to maintain selection.

  • b Liquid MM+U medium was used instead of the recommended liquid RPMI 1640 medium.

  • c C. parapsilosis ATCC 22019. QC, quality control.

  • d TRBR, conidia of the pyrG+ TRB-resistant transformant; rTRBR, conidia derived from retransformation of AF293.1 protoplasts with plasmid obtained from the initial TRBR pyrG+ transformant; TRBR-G, conidia derived from transformation of AF293.1 protoplasts with the complete A. fumigatus squalene epoxidase gene; TRBR-E, conidia derived from transformation of AF293.1 protoplasts with the empty plasmid; TRBR-L, conidia of the strain derived from TRBR after plasmid eviction.