TABLE 1.

pncA nucleotide and amino acid changes in PZA-resistant M. tuberculosis clinical isolates from Portugal

Mutation siteNucleotide changeAmino acid changeaNo. of isolates
−11A→GMutation in promoter2
2T2CMet1→Thr*1
137C137TAla46→Val1
152A152CHis51→Pro1
193A insertionFrameshift1
212A212GHis71→Arg3
250C insertionFrameshift*4
286A286GLys96→Glu3
290T insertionFrameshift*1
359T359CLeu120→Pro*13
374T374GVal125→Gly*8
391GG insertionFrameshift1
395G395AGly132→Asp1
406G406CAsp136→His1
421C421TGln141Stop1
439CG insertionFrameshift*3
476T476CLeu159→Pro*5
511G511AAla171→Thr1
  • a An asterisk indicates a new mutation not reported in previous studies (2, 5, 7, 8, 10, 11, 14-16).