TABLE 1.

Nucleotide and amino acids substitutions in cyp51A and cyp51B genes and MICs of azole drugs for A. fumigatus clinical and mutant isolates

Isolate (strain)bNucleotide substitution in indicated codon of genea:MICs (μg/ml)
cyp51Acyp51BITCVRCRVCPOS
5422035387394
CM-237GGGATGTCTGATCCT0.250.50.50.06
CM-2739GGGATGTCTGATCCT0.500.50.50.06
CM-1369GGGATGTCTGATCCT0.250.50.50.06
CM-1245 (AF-91)GGG 729 GTG* 105TCCGATCCT>8.01.02.0-4.00.5-1.0
CM-1252 (AF-90)GGG 729 GTG* 105TCCGATCCT>8.01.01.0-2.00.5
CM-2158 (AF/1422)GGG 729 GTG* 105TCCGAT 1285CCG>8.01.02.0-4.00.5-1.0
CM-2159 (F/6919)GGG 730AAG**TCTGATCCT>8.01.0-2.01.0-2.02.0
CM-2164 (SO/3829)GGG 730ACG***TCT 1264GAA 1285CCA>8.00.5-1.01.0-2.00.25-0.5
T-XIIGGG 729 GTG*TCTGATCCT>8.01.02.00.5
T-IIIGGG 730AAG**TCTGATCCT>8.01.01.02.0
T-IVGGG 730AAG**TCTGATCCT>8.01.01.01.0
T-VIIGGG 730AAG**TCTGATCCT>8.01.01.01.0
T-XIGGG 730AAG**TCTGATCCT>8.01.01.01.0
T-XXIIGGG 730ACG***TCTGATCCT>8.00.5-1.01.00.12-0.25
  • a Nucleotides are numbered from the translation start codon ATG of cyp51A and cyp51B. The numbers indicate the position at which a base change occurs (indicated in boldface characters). Asterisks indicate the amino acid substitutions for codon 220: *, M220V; **, M220K; ***, M220T.

  • b Transformants T-III, T-VI, T-VII, and T-XI (replacement of mutated cyp51A gene copy from CM-2159), T-XII (cyp51A from CM-1252), and T-XXII (cyp51A copy from CM-2164) are indicated.