TABLE 2.

Primers used in this study

Genetic element(s) or purpose and primerNucleotide sequenceExpected product size (kb)Reference or source
Type V SCCmec of WIS
    V15′-TACCACTTTTACCACTTAGCTTT-3′This study
    V25′-ATGAGGCTTTAACATTTCCATCA-3′This study
    is15′-ACATTAGATATTTGGTTGCGT-3′21
    V35′-TATCATTACACTCTTGAGTCTCT-3′This study
    mA25′-AACGTTGTAACCACCCCAAGA-3′21
    mA35′-TGCTATCCACCCTCAAACAGG-3′21
Detection of ccrC
    γF5′-CGTCTATTACAAGATGTTAAGGATAAT-3′0.52This study
    γR5′-CCTTTATAGACTGGATTATTCAAAATAT-3′This study
Construction of recombinant plasmids with ccrC
    Vcc15′-AAAAGGATCCAAGTTGTTTGCTTAGCGTCATTA-3′1.93This study
    Vcc25′-AAAAGGATCCTAGTACTCATATGATTAAGTGGT-3′This study
attSCC-V (attSCC of type V SCCmec)
    mVR25′-AAAAAGTCGACTACCGTCGATATCAATTGCTTTTT-3′0.90This study
    mVL25′-AAAAAGTCGACTGGAGACGTAGTATAAATATAGCT-3′This study
Detection of precise excision and closed circular DNA formation
    attBscc (N315)
        cL15′-ATTTAATGTCCACCATTTAACA-3′0.2817
        cR15′-AAGAATTGAACCAACGCATGA-3′17
    attBscc (WIS)
        cLs15′-TGCCAATCACAGTTCAATCAATT-3′0.3126
        cR15′-AAGAATTGAACCAACGCATGA-3′17
    attSCC (N315)
        mL15′-GAATCTTCAGCATGTGATTTA-3′0.4622
        mR85′-ATGAAAGACTGCGGAGGCTAACT-3′22
    attSCC (WIS)
        mVL15′-TACTTTGGTTTCATATTAATAGCACT-3′0.29This study
        mVR15′-TCACTAGTGTAATTATCGAATGAT-3′This study
Generation of chromosome-SCCmec (mini-SCC) junction
    α5′-TTTCACACAGGAAACAGCTATGAC-3′22
    β5′-ATCACGATATTGCTTATAAGCA-3′22
    γ5′-ATGTTATTAAGCAGATTGCGTCAA-3′This study