TABLE 1.

K. pneumoniae strains studied and their characteristics

Strain name(s)K. pneumoniae subspeciesGroupa,bpIb,cSourceReference(s)MIC (μg/ml)dbla alleleeBLA amino acid sequencefBeta- lactamaseggyrA alleleemdh alleleeGenBank accession no.
AMPCRXCTXCAZAZT
ATCC 13883T, SB132pneumoniaeKpI7.6Pneumonia, The Netherlands11, 206440.0640.2500.12511SHV-145AJ635407
ATCC 13884TrhinoscleromatisKpI7.6*Nose, Indonesia11, 20320.50.0320.1250.06424New SHV variant310AJ635411
ATCC 11296T, NCTC 5050, SB176ozaenaeKpI7.6Nose, Indonesia11, 20160.50.0160.0320.01632SHV-1145AJ635408
1976 EpneumoniaeKpI*7.6Not known20>256320.1250.2500.06441SHV-149AJ635413
ES694:2pneumoniaeKpI*7.6Feces, Sweden2012880.2500.5000.12551SHV-148AJ635412
HU653:4pneumoniaeKpI*7.6*Feces, Sweden201620.0320.1250.03263SHV-3345AJ635410
MA207:1pneumoniaeKpI*7.6*Feces, Sweden201620.0320.1250.03263SHV-3345AJ635409
UD580:1pneumoniaeKpI*7.6Feces, Sweden203240.0640.1250.01672SHV-1145AJ635415
UD890:1pneumoniaeKpI*7.6Feces, Sweden201680.1250.0640.06482SHV-1149AJ635414
UD1001:4pneumoniaeKpI*7.6Feces, Sweden203220.0320.1250.03272SHV-1145AJ635416
SB18pneumoniaeKpII7.8*Blood, France111620.0320.0640.032914New OKP variant83AJ635401
SB59pneumoniaeKpII8.1*Blood, Spain116420.0640.1250.0641015New OKP variant74AJ635404
LEN-1pneumoniaeKpIII*7.1Feces, Japan20810.0320.0640.016115LEN-111AJ635405
SB1pneumoniaeKpIII7.1*Blood, Austria111620.0320.0640.032127New LEN variant11AJ635400
SB31pneumoniaeKpIII7.1*Blood, Germany111620.0640.1250.032138New LEN variant21AJ635403
SB95 (Kleb PP)pneumoniaeKpIII*7.1*Plant, FranceThis study410.0160.1250.016115LEN-121AJ635405
SB96 (Kleb 701)pneumoniaeKpIII*7.1*Plant, FranceThis study1620.0320.0640.032149New LEN variant21AJ635406
JO436:1pneumoniaeKpIII*7.1Feces, Sweden20820.0640.1250.064157New LEN variant21AJ635421
JO757:2pneumoniaeKpIII*7.1Feces, Sweden201680.0640.2500.064167New LEN variant21AJ635422
KK427:2pneumoniaeKpIII*7.1Feces, Sweden201680.2500.1250.064175LEN-1211AJ635423
OR803:4pneumoniaeKpIII*7.1Feces, Sweden201620.0320.1250.0321810New LEN variant21AJ635417
SO661:2pneumoniaeKpIII*7.1Feces, Sweden20840.0640.0640.0641911New LEN variant11AJ635419
UD711:2pneumoniaeKpIII*7.1Feces, Sweden201640.0640.1250.064206LEN-226AJ635418
VA680:2pneumoniaeKpIII*7.1Feces, Sweden20420.0160.0320.016206LEN-226AJ635426
UD827:1pneumoniaeKpIII*7.1Feces, Sweden20820.0320.1250.0322112New LEN variant21AJ635424
UD892:1pneumoniaeKpIII*7.1Feces, Sweden20820.0640.1250.0642213New LEN variant21AJ635425
SB30pneumoniaeKpII*7.0*Blood, GermanyThis study3240.0640.2500.1252316New OKP variant62AJ635402
OR95:2pneumoniaeKpII*6.5*Feces, Sweden203240.0640.1250.1252417New OKP variant57AJ635420
  • a Phylogenetic group as determined by gyrA and mdh sequencing.

  • b Results determined in this study are marked with an asterisk.

  • c Isoelectric point of chromosomally encoded beta-lactamase.

  • d AMP, ampicillin; CRX, cefuroxime; CTX, cefotaxime; CAZ, ceftazidime; AZT, aztreonam.

  • e The bla, gyrA, and mdh alleles were given arbitrary numbers in this study.

  • f The beta-lactamase amino acid sequences were given arbitrary numbers in this study.

  • g Correspondence with known beta-lactamase amino acid sequences. The new SHV, LEN, and OBK variants are indicated. See Table 3 for amino acid sequences.