TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant featuresaSource or reference
E. coli
    JM109recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB)Laboratory collection
    CH164AG100 ΔmarA zdd-230::Tn917
    SPC105MC4100 containing a chromosomal Pmarn::lacZ fusion at the λ attachment site and a wild-type mar locus10
    BW5104Mu-1 Δlac169 creB510 hsdR51419
    EP663BW5104 tolC::Tn10, Tcr29
E. aerogenes
    ATCC 13048Susceptible strainATCC
    EA27MDR clinical isolate29
Plasmids
    pDriveHigh-copy-number PCR cloning vector, Ampr KnrQiagen
    pRC2pDrive bearing the RaRBS and RaId PCR product to express ramA from PlacThis study
    pBCSK+High-copy-number vector; CmrStratagene
    pRC3EcoRI fragment of pRC2 subcloned into pBCSK+This study
    pmicB21pICIII bearing a micF::lacZ fusion27
  • a Ampr, ampicillin resistant; Knr, kanamycin resistant; Cmr, chloramphenicol resistant, Tcr, tetracycline resistant.