TABLE 2.

Primer sequences

NameSequenceaAnnealing siteRestriction enzyme
GFPf5′-GGG ATCGAT ATGAGTAAAGGA-3′+1 (GFP)ClaI
URA3r5′-AAA CTGCAG AAGGACCACCTTT-3′+924 (URA3)PstI
5′CDR4f5′-CCC GGTACC GGCTAGCAGTTTGAG-3′−325 (CDR4)KpnI
5′CDR4r5′-GG CTCGAG CGCATCTGCTGCAGG-3′+1764 (CDR4)XhoI
3′CDR4f5′-CACCTGCAGGGGTGCAATGCA-3′+2366 (CDR4)None
3′CDR4r5′-CCCG AGCTC CAACGTTTACGTCT-3′+4458 (CDR4)SacI
MDR1pf5′-CAA CTCGAG CACACAGCCGTGAATCTTA-3′−5421 (CaMDR1)XhoI
MDR1pr5′-CCC ATCGAT GTGAAGTTCTATGTAAGTAGATG-3′−1 (CaMDR1)ClaI
CDR1pf5′-CC CTCGAG GTTACTCAATAAGTATTAA-3′−1182 (CDR1)XhoI
CDR1pr5′-CC ATCGAT AATTTTTTTCTTTTTGACC-3′−1 (CDR1)ClaI
CDR2pf5′-CC CTCGAG ACTAGAAGGTTATCAAGA-3′−866 (CDR2)XhoI
CDR2pr5′-CCC ATCGAT ATGTTTTTATTGTATGTG-3′−1 (CDR2)ClaI
ACT1pf5′-CC CTCGAG AGAGCTATTAAGATCACCAG-3′−1017 (ACT1)XhoI
ACT1pr5′-CC ATCGAT TTTGAATGATTATATTTTTTT-3′−1 (ACT1)ClaI
E5′CCACATCAGGAGATTATTCGAGCT-3′−399 (CDR4)None
B5′-ACATCACCATCTAATTCAACAAG-3′+42 (GFP)None
C′5′-TTTCCTATGAATCCACTATTGAACC-3′+208 (URA3)None
F5′-TCCCTGATAGCACTTGGCATTTAT-3′+4630 (CDR4)None
G5′-GGAGTTCTTCGGAAATTGACCATAAAA-3′+1847 (CDR4)None
  • a Recognition sequences used for the ligation of PCR products are shown in italic type. Sequences correspond to genes of C. albicans, except for the yEGFP gene from Aqueroea victoria (19) and hisG for Salmonella enterica serovar Typhimurium ATP phosphoribosyltransferase (9, 26).