TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaReference or source
A. baumannii
    BM4454Wild strain, MDR 22
    BM4542BM4454 adeB::pAT794 22
    BM4543BM4454 adeC::pAT798, MDRThis work
    BM4544BM4454 adeR::pAT799This work
    BM4545BM4454 adeS::pAT800, MDRThis work
    CIP 70-10Reference strain 10
    BM4546CIP 70-10 AdeST153M spontaneous mutant, MDRThis work
    BM4547CIP 70-10 AdeRP116L spontaneous mutant, MDRThis work
    BM4548Wild strainThis work
Plasmid
    pUC18Tra Mob Ap, lacZα 36
    pAT794pUC18Ω979-bp fragment of adeB from BM4454 22
    pAT798pUC18Ω630-bp fragment of adeC from BM4454This work
    pAT799pUC18Ω375-bp fragment of adeR from BM4454This work
    pAT800pUC18Ω621-bp fragment of adeS from BM4454This work
    pKK232-8Tra Mob Ap, cat promoterless 11
    pAT804pKK232-8Ω809-bp fragment upstream from adeCThis work
    pAT805pKK232-8Ω228-bp fragment upstream from adeCThis work
  • a Tra, non-self-transferable; Mob, nonmobilizable; Ap, ampicillin resistance, cat, chloramphenicol resistance gene; MDR, multidrug resistance due to overexpression of the AdeABC pump.