TABLE 6.

Effect of early adherence on gene expression

StrainConstructMean ± SD relative fluorescence (FL1)a (arbitrary units; logarithmic scale) of the following cells:
PlanktonicAdherentAdherent cell/planktonic cell ratio
CaI4Parental47.71 ± 0.6251.89 ± 0.921
CaCMR5 CaMDR1 promoter-yEGFP98.89 ± 17103.43 ± 11.30.95
CaCMR4 ACT1 promoter-yEGFP43.34 ± 0.3393.56 ± 6.622.2
CaCMR7 CDR1 promoter-yEGFP42.29 ± 1.04220.03 ± 3.345.2
CaCMR8 CDR2 promoter-yEGFP44.63 ± 0.4573.9 ± 3.351.65
  • a From three independent experiments. The Student t test reflected P values of 0.005 for CaCMR1, 0.0001 for CDR1, and 0.005 for CDR2, indicating 99% confidence.