TABLE 7.

yEGFP expression in daughter cells

Cell populationaMean ± SD relative fluorescence (FL1)b (arbitrary units; logarithmic scale) at:
24 h48 h
CaCMR4 planktonic46 ± 783 ± 8c
CaCMR4 daughter50 ± 4.148 ± 8.8c
CaCMR4 detached49 ± 3.791.5 ± 13.7c
CaCMR7 planktonic141 ± 281,509 ± 93.2c
CaCMR7 daughter118 ± 9.8160 ± 34.3c
CaCMR7 detached162.4 ± 6.61,765 ± 301c
CaCMR8 planktonic53 ± 6.594 ± 12
CaCMR8 daughter50 ± 13.696 ± 5.2
CaCMR8 detached50 ± 4.891.5 ± 14
  • a Daughter, planktonic cells that were born from mature biofilms; detached, planktonic cells that were allowed to adhere to medical-grade silicone disks for 2 h prior to detachment.

  • b From four independent experiments.

  • c Results were statistically significantly different (P = 0.031 for CaCMR4 cells and P = 0.004 for CaCMR7 cells) between daughter cells and detached cells or planktonic cells of the same strain.