TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionaReference or source
E. coli DH10BF mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara, leu)7697 araD139 galU galK nupG rspL λ12
E. faecalis
    JH2-2Type strain; Rfr Fsr13
    AR01/DGStrain isolated from a dog with mastitis; harbors pJM01 (Tcr Bcr) and pJM02 (Vmr Emr)20
    AR01/DGVSAR01/DG cured of pJM02; Tcr BcrThis study
    DGM2AR01/DGVS bcrB::Tn917; Emr Bcs TcrThis study
    DGM4AR01/DGVS bcrA::Tn917; Emr Bcs TcrThis study
    AR01/DGVS/pTV1-OKAR01/DGVS harbouring pTV1-OKThis study
    DGM2/pAMBcr1DGM2 harboring pAMBcr1This study
    DGM4/pAMBcr1DGM4 harboring pAMBcr1This study
    JH2-2/pAMBcr1JH2-2 harboring pAMBcr1This study
    JH2-2/pAMBcr2JH2-2 harboring pAMBcr2This study
    JH2-2/pAMBcr3JH2-2 harboring pAMBcr3This study
    JH2-2/pMGSBcr4JH2-2 harboring pMGSBcr4This study
    JH2-BcrJH2-2 harboring pJM01; Tcr BcrThis study
Plasmids
    pUC8Cloning vector; Apr36
    p2H7pUC8 harboring 4.7-kb EcoRI fragment from AR01/DGVS containing bcrR, bcrA, bcrB; and bcrDThis study
    pTV1-OKrepA(Ts)-pWV01Ts aphA3 Tn917; Kmr Emr11
    pAM401E. coli-E. faecalis shuttle vector; Cmr Tcr35
    pMGS100E. coli-E. faecalis shuttle expression vector; Cmr9
    pAMBcr1pAM401 harboring 4.7-kb EcoRI fragment from p2H7 containing bcrR, bcrA, bcrB, and bcrDThis study
    pAMBcr2pAM401 harboring 3.6-kb EcoRV-SspI fragment from p2H7 containing bcrA, bcrB, and bcrDThis study
    pAMBcr3pAM401 harboring 3.2-kb EcoRI-NdeI fragment from p2H7 containing bcrR, bcrA, and bcrBThis study
    pMGSBcr4pMGS100 harboring bcrDThis study
  • a Bcs, sensitivity to bacitracin; r, resistant; s, sensitive.