TABLE 3.

Features and/or open reading frames of the multidrug resistance region of pC15-1a

LocationFeature(s) and/or open reading frame(s)Gene(s)% HomologyaGenBank accession no.
1-3664Tn5403; transposase, resolvasetnpA, tnpR99.8X75779
3663-9641Central core of Tn1721 (bp 4091-10096); partial transposases, PecM-like transcriptional regulator, tetracycline resistance protein, tetracycline resistance repressortnpA partial, pecM-like, tetA, tetR99.4X61367
9642-10461IS26tnpA100AF550679
10462-10499Partial chloramphenicol O-acetyltransferasecatB3100AJ310778
10718-11548Beta-lactamaseblaOXA-1100AF326777
11679-12278Aminoglycoside N6′-acetyltransferaseaac(6′)-Ib100AY259086
12325-13144IS26tpnA100AF550679
13145-13475Partial chloramphenicol O-acetyltransferasecatB3100AJ310778
13476-14295IS26tnpA100AF550679
14339-15199Aminoglycoside N3′-acetyltransferaseaac(3)-II100AY138987
15212-15754Hypothetical; identical to hypothetical protein AAN34369 in Acinetobacter baumanniihypo100AY138987
15825-16157IS3 family transposase; Agrobacterium tumefaciens C58 OrfAorfA95.1 (aa)NP356057
16154-16894Partial; putative transposase protein of Sinorhizobium melilotiorfB89.5 (aa)NP387207
16896-17715IS26tnpA99.6AF550679
17716-19960Tn3-like partial transposase; disrupted by ISEcp1/CTX-M transposition elementtnpA100AB103092
20353-21228Beta-lactamaseblaCTX-M-15100AY044436
21277-22932ISEcp1tnpA100AY044436
22933-23146Tn3-like partial transposase; disrupted by ISEcp1/CTX-M transposition elementtnpA100AB103092
23145-25057Tn3-like resolvase, beta-lactamasetnpR, blaTEM-199.7AB103092
25057-27528Partial transposase, identical to Tn21 tnpA from R100tnpA100NC_002134
27531-27628Partial resolvase, identical to Tn21 tnpR from R100tnpR100NC_002134
27619-28438IS26tnpA100AF550679
  • a Unless otherwise indicated, percent homologies are bases on nucleic acid comparisons. Amino acid (aa) homologies were used if they proved to be more informative of gene function. In many cases, numerous BLAST hits were obtained, all with similar degrees of homology. In such cases, only one was selected for reference purposes.