TABLE 3.

Features and/or open reading frames of the multidrug resistance region of pC15-1a

LocationFeature(s) and/or open reading frame(s)Gene(s)% HomologyaGenBank accession no.
1-3664Tn5403; transposase, resolvase tnpA, tnpR99.8 X75779
3663-9641Central core of Tn1721 (bp 4091-10096); partial transposases, PecM-like transcriptional regulator, tetracycline resistance protein, tetracycline resistance repressor tnpA partial, pecM-like, tetA, tetR99.4 X61367
9642-10461IS26 tnpA 100 AF550679
10462-10499Partial chloramphenicol O-acetyltransferase catB3 100 AJ310778
10718-11548Beta-lactamase bla OXA-1 100 AF326777
11679-12278Aminoglycoside N6′-acetyltransferase aac(6′)-Ib100 AY259086
12325-13144IS26 tpnA 100 AF550679
13145-13475Partial chloramphenicol O-acetyltransferase catB3 100 AJ310778
13476-14295IS26 tnpA 100 AF550679
14339-15199Aminoglycoside N3′-acetyltransferase aac(3)-II100 AY138987
15212-15754Hypothetical; identical to hypothetical protein AAN34369 in Acinetobacter baumanniihypo100 AY138987
15825-16157IS3 family transposase; Agrobacterium tumefaciens C58 OrfAorfA95.1 (aa) NP356057
16154-16894Partial; putative transposase protein of Sinorhizobium melilotiorfB89.5 (aa) NP387207
16896-17715IS26 tnpA 99.6 AF550679
17716-19960Tn3-like partial transposase; disrupted by ISEcp1/CTX-M transposition element tnpA 100 AB103092
20353-21228Beta-lactamase bla CTX-M-15 100 AY044436
21277-22932ISEcp1 tnpA 100 AY044436
22933-23146Tn3-like partial transposase; disrupted by ISEcp1/CTX-M transposition element tnpA 100 AB103092
23145-25057Tn3-like resolvase, beta-lactamase tnpR, blaTEM-199.7 AB103092
25057-27528Partial transposase, identical to Tn21 tnpA from R100 tnpA 100 NC_002134
27531-27628Partial resolvase, identical to Tn21 tnpR from R100 tnpR 100 NC_002134
27619-28438IS26 tnpA 100AF550679
  • a Unless otherwise indicated, percent homologies are bases on nucleic acid comparisons. Amino acid (aa) homologies were used if they proved to be more informative of gene function. In many cases, numerous BLAST hits were obtained, all with similar degrees of homology. In such cases, only one was selected for reference purposes.