TABLE 1.

Peptide sequences of SHV-1 β-lactamase recognized by the anti-SHV-1 antibodya

Recognized amino acids in SHV-1Sequence% Signal intensity
26-38S P Q P L E Q I K L S E S34
28-40Q P L E Q I K L S E S Q L32
Consensus 28-38Q P L E Q I K L S E S
TEM sequenceE T L V K V K D A E D
36-48S E S Q L S G R V G M I E14
38-50S Q L S G R V G M I E M D28
40-52L S G R V G M I E M D L A62
42-54G R V G M I E M D L A S G65
44-56V G M I E M D L A S G R T50
46-58M I E M D L A S G R T L T34
Consensus 42-54G R V G M I E M D L A S G
TEM sequenceA R V G Y I E L D L N S G
88-100D E Q L E R K I H Y R Q Q80
90-102Q L E R K I H Y R Q Q D L56
92-104E R K I H Y R Q Q D L V D29
94-106K I H Y R Q Q D L V D Y S21
Consensus 88-100D E Q L E R K I H Y R Q Q
TEM sequenceQ E Q L G R R I H Y S Q N
98-110R Q Q D L V D Y S P V S E48
100-112Q D L V D Y S P V S E K H55
102-114L V D Y S P V S E K H L A61
104-116D Y S P V S E K H L A D G59
Consensus 102-114L V D Y S P V S E K H L A
TEM sequenceL V E Y S P V T E K H L T
164-176R W E T E L N E A L P G D64
166-178E T E L N E A L P G D A R81
168-180E L N E A L P G D A R D T96
170-182N E A L P G D A R D T T T100
172-184A L P G D A R D T T T P A89
174-186P G D A R D T T T P A S M80
176-188D A R D T T T P A S M A A35
Consensus 170-182N E A L P G D A R D T T T
TEM sequenceN E A I P N D E R D T T M
182-194T P A S M A A T L R K L L19
184-196A S M A A T L R K L L T S20
186-198M A A T L R K L L T S Q R22
188-200A T L R K L L T S Q R L S8
190-202L R K L L T S Q R L S A R9
Consensus 186-194M A A T L R K L L
TEM sequenceM A T T L R K L L
198-210R L S A R S Q R Q L L Q W28
200-212S A R S Q R Q L L Q W M V31
202-214R S Q R Q L L Q W M V D D31
Consensus 202-210R S Q R Q L L Q W
TEM sequenceA S R Q Q L I D W
276-288N Q Q I A G I G A A L I E21
Consensus 276-288N Q Q I A G I G A A L I E
TEM sequenceN R Q I A E I G A S L I K
  • a The percent signal intensity for each of the recognized peptide spots, regions of consensus in each of the recognized peptides of SHV-1 β-lactamase, and the corresponding regions for TEM-1 β-lactamase are shown. Standard deviations for signal intensity percentages never exceeded 8% of the reported values. Boldface indicates region of identical amino acids between peptides. Boldface italics indicates peptide consensus region of SHV-1 β-lactamase and corresponding identical amino acids in TEM-1 β-lactamase.