TABLE 2.

Peptide sequences of CMY-2 β-lactamase recognized by the anti-CMY-2 (AmpC) antibodya

Recognized amino acids in CMY-2Sequence% Signal intensity
1-13 A A K T E Q Q I A D I V N 100
4-16 T E Q Q I A D I V N R T I 98
7-19 Q I A D I V N R T I T P L 87
10-22 D I V N R T I T P L M Q E 73
CMY-2 aa 4-16 T E Q Q I A D I V N R T I
AmpC C. freundii T E Q Q I A D I V N R T I
P99 E. cloacae S E K Q L A E V V A N T I
AmpC M. morganii A A D N V A A V V D S T I
67-79 K T F N G V L G G D A I A 25
70-82 N G V L G G D A I A R G E 23
CMY-2 aa 70-79 N G V L G G D A I A
AmpC C. freundii N G V L G G D A I A
P99 E. cloacae T G V L G G D A I A
AmpC M. morganii T G V L G A V S V A
208-220 P V H V S P G Q L D A E A 83
211-223 V S P G Q L D A E A Y G V 97
214-226 G Q L D A E A Y G V K S S 81
CMY-2 aa 211-223 V S P G Q L D A E A Y G V
AmpC C. freundii V S P G Q L D A E A Y G V
P99 E. cloacae V S P G M L D A Q A Y G V
AmpC M. morganii V S P G Q L D A E S Y G V
271-283 W E M L N W P L K A D S I 24
274-286 L N W P L K A D S I I N G 29
277-289 P L K A D S I I N G S D S 32
CMY-2 aa 274-286 L N W P L K A D S I I N G
AmpC C. freundii L N W P V K A D S I I S G
P99 E. cloacae L N W P V E A N T V V E G
AmpC M. morganii Y D W P Q Q L D M I I N G
286-298 G S D S K V A L A A L P A 50
289-301 S K V A L A A L P A V E V 60
CMY-2 aa 289-298 S K V A L A A L P A
AmpC C. freundii S K V A L A A L P A
P99 E. cloacae S K V A L A P L P V
AmpC M. morganii N E V A L Q P H P V
319-331 T G G F G S Y V A F V P E 25
322-334 F G S Y V A F V P E K N L 32
325-337 Y V A F V P E K N L G I V 33
328-340 F V P E K N L G I V M L A 26
CMY-2 aa 322-334 F G S Y V A F V P E K N L
AmpC C. freundii F G S Y V A F V P E K N L
P99 E. cloacae F G S Y V A F I P E K N I
AmpC M. morganii F G A Y V A F I P E K N V
340-352 A N K S Y P N P V R V E A 17
343-355 S Y P N P V R V E A A W R 18
346-358 N P V R V E A A W R I L E 38
CMY-2 aa 343-358 S Y P N P V R V E A A W R I L E
AmpC C. freundii S Y P N P V R V E A A W R I L E
P99 E. cloacae S Y P N P A R V E A A Y H I L E
AmpC M. morganii N T P N T E R V K A A Q A I L S
  • a CMY-2 immobilized synthetic peptide sequences recognized by the antibody and their corresponding amino acid positions in CMY-2. The percent signal intensity for each of the recognized peptide spots, regions of consensus in each of the recognized peptides of CMY-2 β-lactamase, and the corresponding consensus region for the AmpC β-lactamase of C. freundii, P99 E. cloacae, and AmpC of M. morganii are shown. Standard deviations for signal intensity percentages never exceeded 8% of the reported values. Boldface indicates region of identical amino acids between peptides. Boldface italics indicates peptide consensus region of CMY-2 β-lactamase and corresponding identical amino acids in related AmpC β-lactamases.