TABLE 2.

Peptide sequences of CMY-2 β-lactamase recognized by the anti-CMY-2 (AmpC) antibodya

Recognized amino acids in CMY-2Sequence% Signal intensity
1-13A A K T E Q Q I A D I V N100
4-16T E Q Q I A D I V N R T I98
7-19Q I A D I V N R T I T P L87
10-22D I V N R T I T P L M Q E73
CMY-2 aa 4-16T E Q Q I A D I V N R T I
AmpC C. freundiiT E Q Q I A D I V N R T I
P99 E. cloacaeS E K Q L A E V V A N T I
AmpC M. morganiiA A D N V A A V V D S T I
67-79K T F N G V L G G D A I A25
70-82N G V L G G D A I A R G E23
CMY-2 aa 70-79N G V L G G D A I A
AmpC C. freundiiN G V L G G D A I A
P99 E. cloacaeT G V L G G D A I A
AmpC M. morganiiT G V L G A V S V A
208-220P V H V S P G Q L D A E A83
211-223V S P G Q L D A E A Y G V97
214-226G Q L D A E A Y G V K S S81
CMY-2 aa 211-223V S P G Q L D A E A Y G V
AmpC C. freundiiV S P G Q L D A E A Y G V
P99 E. cloacaeV S P G M L D A Q A Y G V
AmpC M. morganiiV S P G Q L D A E S Y G V
271-283W E M L N W P L K A D S I24
274-286L N W P L K A D S I I N G29
277-289P L K A D S I I N G S D S32
CMY-2 aa 274-286L N W P L K A D S I I N G
AmpC C. freundiiL N W P V K A D S I I S G
P99 E. cloacaeL N W P V E A N T V V E G
AmpC M. morganiiY D W P Q Q L D M I I N G
286-298G S D S K V A L A A L P A50
289-301S K V A L A A L P A V E V60
CMY-2 aa 289-298S K V A L A A L P A
AmpC C. freundiiS K V A L A A L P A
P99 E. cloacaeS K V A L A P L P V
AmpC M. morganiiN E V A L Q P H P V
319-331T G G F G S Y V A F V P E25
322-334F G S Y V A F V P E K N L32
325-337Y V A F V P E K N L G I V33
328-340F V P E K N L G I V M L A26
CMY-2 aa 322-334F G S Y V A F V P E K N L
AmpC C. freundiiF G S Y V A F V P E K N L
P99 E. cloacaeF G S Y V A F I P E K N I
AmpC M. morganiiF G A Y V A F I P E K N V
340-352A N K S Y P N P V R V E A17
343-355S Y P N P V R V E A A W R18
346-358N P V R V E A A W R I L E38
CMY-2 aa 343-358S Y P N P V R V E A A W R I L E
AmpC C. freundiiS Y P N P V R V E A A W R I L E
P99 E. cloacaeS Y P N P A R V E A A Y H I L E
AmpC M. morganiiN T P N T E R V K A A Q A I L S
  • a CMY-2 immobilized synthetic peptide sequences recognized by the antibody and their corresponding amino acid positions in CMY-2. The percent signal intensity for each of the recognized peptide spots, regions of consensus in each of the recognized peptides of CMY-2 β-lactamase, and the corresponding consensus region for the AmpC β-lactamase of C. freundii, P99 E. cloacae, and AmpC of M. morganii are shown. Standard deviations for signal intensity percentages never exceeded 8% of the reported values. Boldface indicates region of identical amino acids between peptides. Boldface italics indicates peptide consensus region of CMY-2 β-lactamase and corresponding identical amino acids in related AmpC β-lactamases.