Comparison of biochemical properties of Ser1-PMP-1, Asp1-PMP-1, and hPF-4
Peptide | Tissue source | Sequence source | N-terminal 10-amino-acid sequence | Length (no. of residues) | Calculated mass (Da)a | Actual mass (Da)a |
---|---|---|---|---|---|---|
Pre-PMP-1 | Bone marrow | cDNA | MSIPEASGAP | 106 | 11,156 | NDb |
Ser1-PMP-1 | Resting platelet (acid extract) | Amino acid | SDDPKESEGD | 73 | 8,044 | 8,053 |
Asp1-PMP-1 | Activated platelet (thrombin stimulated) | Amino acid | DDPKESEGDL | 72 | 7,957 | 7,951 |
Pre-hPF-4 | Erythroleukemic cell line | cDNA | MSSAAGFCAS | 101 | 10,838 | ND |
hPF-4 | Activated platelet | Amino acid | AEAEEDGDLQC | 70 | 7,765 | 7,756 |