TABLE 3.

Plasmids used in this study

PlasmidDescriptionSource or reference
B2159 ADE3 URA3 CEN plasmidG. Fink laboratory collection
pRS305 LEU2 2μm plasmid 31
pRS315 LEU2 CEN plasmid 31
pRS426 URA3 2μm plasmid 1
YIp363β-Gal reporter LEU2-integrating plasmid 22
pGEM-HIS1 CaHIS1-integrating plasmid 35
pRS-ARG4ΔSpeI CaARG4 CEN plasmid 35
pDDB57 CaURA3-dpl200 cassette 34
pGFP-HIS1 GFP-CaHIS1 cassette 7
LOB421.2-kb CDR1 promoter-lacZ in- frame fusion in YIp363This study
LOB431.6-kb ADE3 in LOB42This study
LOB44 CaNDT80 URA3 2μm plasmidThis study
LOB452.8-kb full length CaNDT80 URA3 2μm PRS426 plasmidThis study
LOB46 NDT80 URA3 2μm plasmidThis study
LOB47 GFP-CaARG4 cassetteThis study
LOB48 tetR-ScHAP4-3xHA CaHIS1- encoding plasmidThis study
LOB49 CaNDT80 CaHIS1-integrating plasmidThis study