TABLE 1.

MICs of CA(1-8)M(1-18) and PXB for A. baumannii at 107 CFU/ml under different experimental conditions

AgentaMIC (μM)
CA(1-8)M(1-18)PXB
Standard conditions (Hanks+Glc)21
NaCl (mM)
        2710.25
        8120.5
        13621
Divalent cations
        Ca2+ (mM)
            0.0521
            1.082
            5.0164
        Mg2+ (mM)
            0.0521
            1.082
            5.0164
EDTA (mM)
            0.2510.5
            1.00.50.25
            5.00.250.12
Polyanionsb
        Heparin (mg/ml)
            0.0121
            0.182
            1.0162
        Alginate (mg/ml)
            0.0141
            0.1162
            1.0>162
Uncoupler CCCP (μM)b
        12.524
        5044
        10048
  • a Unless otherwise stated, bacteria were preincubated with the corresponding agent for 15 min prior to peptide addition.

  • b Peptide and the corresponding agent were preincubated for 15 min before being added to the bacterial suspension. CCCP, carbonyl cyanide m-chlorophenylhydrazone.