TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeSource or reference
Strains
        E. coli DH10BaraD139 Δ(ara,leu)7697 deoR endA1 galK1 galU nupG recA1 rpsL F" mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15ΔlacX74 SmrGibco BRL
        E. coli JM109endA1 gyrA96 hsdR17 Δ(lac proA) relA recA1 supE44 thi F" (lacIq lacZΔM15 proAB+ traD36)Promega
        E. coli HB101F-mcrB mrr hsdS20(rB mB) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 (Smr) supE44 λGibco BRL
        In vitro-obtained rifampin-and nalidixic acid-resistant E. coli K-12Rifr NalrThis study
        In vitro-obtained rifampin-resistant P. aeruginosa ATCC 27853RifrThis study
        P. aeruginosa Pa695Extended-spectrum cephalosporin-and aminoglycoside-resistant clinical isolateThis study
Plasmids
        pBK-CMV phagemidKanr NeorStratagene, Inc.
        pGEM-TAmprPromega
        pTK1Natural plasmid from K. pneumoniae ORI-1 containing blaGES-1 and aac(6")-Ib" genes32
        pC18Recombinant pBK-CMV plasmid with a 2.86-kb genomic Sau3AI fragment containing blaGES-1This study
        pC23Recombinant pBK-CMV plasmid with a 18.5-kb genomic Sau3AI fragment containing blaGES-1This study
        pI182,390-bp PCR fragment containing the pC18 insert in pGEM-TThis study
        pA3A61,104-bp PCR fragment containing the aac(3)-Ib/aac(6")-Ib" gene from pC18 in pGEM-TThis study
        pA3541-bp PCR fragment containing the aac(3)-Ib gene from pC18 in pGEM-TThis study
        pA6588-bp PCR fragment containing the aac(6")-Ib" gene from pC18 in pGEM-TThis study
        pA6In52588-bp PCR fragment containing the aac(6")-Ib" gene from pTK1 in pGEM-TThis study