TABLE 1.

Relevant characteristics of bacterial strains and plasmids

Strain or plasmidRelevant characteristicsSourcea or reference
Strains
    E. coli
        DH5αMCRFmcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 Δ(lacZYA-argF) U169 deoR recA1 endA1 thi-1 phoA supE44 gyrA96 relA1 λGibco-BRL
        LE392F e14mcrA hsdR514(rK mK+) supE44 supF58 lacY1 galK2 galT22 metB1 trpR55Promega
        S17-1 recA pro hsdR RP4-2-Tc::Mu-Kan::Tn7 Tmpr Sptr Strr29
    A. pyogenes
        BBR1Bovine isolate, Tetr6
        JGS190Bovine isolate, TetrCSU
        3Bovine isolate, Tetr25
        4Bovine isolate, Tetr25
        98-1508Macaw isolate, TetrAZ VDL
        3274Bovine isolate, TetsCSU
        JGS478 nanP::Kan derivative of strain 3274This study
        JGS603Tetr Kanr BBR1 × JGS478 transconjugantThis study
        JGS605Tetr Kanr JGS190 × JGS478 transconjugantThis study
        JGS610 nanH::Erm derivative of strain 3274This study
Plasmids
    pBC KSChlr lacZ′, ColE1 originStratagene
    pCR-Script Cam SK(+)pBC SK with incorporated SrfI siteStratagene
    pWSK129KanrlacZ′, p15A origin38
    pJRD215Kanrmob+, RSF1010 origin12
    pJGS259pBC KS BamHI Ω 4-kb partial Sau3AI fragment containing the tet(W) gene of strain 4This study
    pJGS264pJGS259 Δ 1.3-kb NotI fragmentThis study
    pJGS279pBC KS BamHI Ω 12-kb BamHI fragment from λJGS9 containing the tet(W) gene of BBR1This study
    pJGS324pCR-Script Cam SK SfiI Ω 1,710-bp PCR product containing mob and oriTThis study
    pJGS416pBC KS SacI-KpnI Ω 1,524-bp PCR product containing mobThis study
    pJGS417pWSK129 SacI-KpnI Ω 202-bp PCR product containing oriTThis study
  • a CSU, Colorado State University; AZ VDL, Arizona Veterinary Diagnostic Laboratory.