TABLE 1.

Strains and plasmids used in the study

Strain or plasmidRelevant characteristicsaSource or reference
E. coli
    JM83araΔ(lac-proAB)rpsL(Strr)[F80dlacΔ(lacZ)M15]38
    C600Rife14-(mcrA)thr-1 leuB6 thi-1 lacY1 supE44 rfbD1 fhuA2124
    HB101hsdS20 Δ(gpt-proA)62 leu supE44-ara-14 galK2 lacY1 Δ(merC-mrr) rpsL20 (Strr)xyl-5 mtl-1 recA1337
    DH5α(φ80ΔlacZΔM15) endA1 recA1 hsdR17 supE44 thi-1 λ gyrA96 relA1 Δ(lacZYA-argF)U16943
K. pneumoniae
    BM4493Ctxr Cmr Gmr Ntr Smr Sur Tmr TprWild-type strain
    BM4494Ctxr Cmr Gmr Rifr Smr Sur TmrWild-type strain
Plasmids
    pBGS18Tra Mob Kmr39
    pIP843Tra Mob+ Ctxr blaCTX-M-17From BM4493
    pIP843-1Tra Mob Ctxr blaCTX-M-17 (IS903-CΩ4bp)This work
    pIP844Tra Mob Ctxr blaCTX-M-17From BM4494
    pAT797pBGS18Ω 4-kb HindIII from pIP844, Ctxr KmrThis work
    pOX38Tra+ IncF1 Gmr11
  • a Abbreviations: Tra+, self-transferable; Tra, non-self-transferable; Mob+, mobilizable; Mob, nonmobilizable; Apr, ampicillin resistance; Ctxr, cefotaxime resistance; Cmr, chloramphenicol resistance; Gmr, gentamicin resistance; Kmr, kanamycin resistance; Ntr, netilmicin resistance; Rifr, rifampin resistance; Smr, streptomycin resistance; Sur, sulfonamide resistance; Tmr, tobramycin resistance; Tpr, trimethoprim resistance.