TABLE 1.

Plasmids

PlasmidDescriptionRelevant phenotypeaReference
pMO266R18-18::Tn2521Apr Cbr Kmr Smr Spr Sur Tcr tra+35
R38833 kb IncW plasmid containing In3 (dfrB2-orfA cassettes)Sur Tpr tra+1
R388::Tn1405Tn1405 “transposed” into R388Apr Cbr Smr Spr Sur24
pRMH1615.7-kb SalI fragment of pMO266 in pUC19Apr Smr Spr SurThis work
pRMH1622.2-kb + 0.16-kb HindIII fragments of pMO266 in pUC19Apr SurThis work
pRMH1645.3-kb HindIII fragment of pMO266 in pUC19AprThis work
pRMH4863.4-kb HindIII-KpnI fragment of pRMH164 in pUC19AprThis work
pRMH4892.4-kb PstI fragment of pRMH486 in pUC19AprThis work
pRMH4900.7-kb PstI fragment of pRMH486 in pUC19AprThis work
pRMH560Cassette-free derivative of R388Sur tra+29
pRMH8584.8-kb BamHI fragment of R388::Tn1405 in pACYC184Apr Cmr Smr Spr SurThis work
  • a Ap, ampicillin; Cb, carbenicillin; Cm, chloramphenicol; Km, kanamycin; Sm, streptomycin; Sp, spectinomycin; Su, sulfonamide; Tc, tetracycline; Tp, trimethoprim.