TABLE 1.

Bacterial strains used in this study

Strain (characteristic[s])ApplicationReference or source
P. aeruginosa 101/1477 (IMP-1 producer)Positive controlD. M. Livermore
P. aeruginosa NCTC 50814 (met lys his Rif)RecipientNCTCa
E. coli UB1637/R (his lys trp Rif)Recipient 4
P. aeruginosa ATCC 27853Quality controlATCCb
E. coli ATCC 25922Quality controlATCC
Agrobacterium tumefaciens C58(pAtC58 [543 kb]/pTiC58 [214 kb])Plasmid size 6
Rhizobium leguminosarum 3841 (147, 151, 350, 488, 684, and 870 kb)Plasmid size http://www.sanger.ac.uk/Projects/Microbes/
Rhizobium leguminosarum VF39 (150, 220, 400, 500, 700, and 900 kb)Plasmid size 9
Rhizobium leguminosarum LRS39401 (150, 220, 400, 700, and 900 kb)Plasmid size 9
P. aeruginosa PAO1 (IncP2 plasmid pBS31 [400 kb])IncP testC. M. Thomas
P. putida ML4262 (trp his) (IncP9 plasmid R2 [68 kb])IncP testC. M. Thomas
P. putida ML4262 (trp his) (IncP2 plasmid pBS228 [130 kb])IncP testC. M. Thomas
P. aeruginosa PU21 (ilvB112 leu-1 str-1 Rifr)IncP testG. A. Jacoby
P. aeruginosa PAO2003 (arg-32 str-39 rec-2)IncP testG. A. Jacoby
P. aeruginosa PAO2003(CAM) (camphor plasmid)IncP testG. A. Jacoby
  • a NCTC, National Collection of Type Cultures.

  • b ATCC, American Type Culture Collection.