TABLE 2.

Primer pairs used for producing PCR fragments

FragmentPrimerSize (bp)Resulting plasmid
ForwardReverse
lacZ fragment for creating fusionslacF2-BamHIlacR1-PstIpVRASRerm-pCL52.1
vraSR operon fragmentsvraSR-FvraSR-R1,700pVRASRerm
    vraSR probevraSRprobeFvraSRprobeR2,022None (not cloned)
    VP1VraF1-KpnIVraR1-BamHI1,183pVP1
    VP1′VraF2-KpnIVraR1-BamHI1,513pVP1′
    VP2VraF3-KpnIVraR2-BamHI1,076pVP2
    VP2′VraF4-KpnIVraR2-BamHI2,218pVP2′
    VP-262VraF5-KpnIVraR3-BamHI412pVP-262
    VP-462VraF6-KpnIVraR3-BamHI612pVP-462
pbp2 operon fragments
    PBP2-aPBP2F1-KpnIPBP2R1-PstI326pPBP2-a
    PBP2-bPBP2F1-KpnIPBP2R2-PstI955pPBP2-b
    PBP2-cPBP2F2-KpnIPBP2R2-PstI647pPBP2-c
    PBP2-dPBP2F3-KpnIPBP2R2-PstI431pPBP2-d