RFLP analysis of the sequences at positions 116 and 266 in ERG11a
| Amino acid present | Isolate(s) resistant to the following no. of azoles: | ||||
|---|---|---|---|---|---|
| Four | Three | Two | One | None | |
| 116D/D | 2, 5, 8, 9 | 15, 18, 20, 21 | 22, 23, 30 | 31, 32 | |
| 116D/E | 1, 3, 4, 7, 10 | 13, 14 | 17, 19 | 24, 25, 26, 27, 28, 29 | 33, 34, 35, 36 |
| 116E/E | 6 | 11, 12 | 16 | ||
| 266E/E | 1, 2, 3, 5, 6, 7, 8, 9, 10 | 11, 13, 14 | 15, 17, 18, 19, 21 | 22, 24, 25, 26, 27, 28, 30 | 31, 33, 34 |
| 266E/D | 4 | 16, 20 | 29 | 36 | |
| 266D/D | 12 | 23 | 32, 35 | ||
↵a RFLP analysis was performed with the 36 isolates to assess the sequences at two locations, corresponding to amino acids 116 and 266, as described in Materials and Methods. The possible amino acids at those positions can be distinguished by RFLP analysis.