TABLE 3.

Site-directed mutagenesisa

MutationIC50Fold change
wt0.24
G36D1.45.8
G36D/Q32R1.35.4
V38A/Q32R1.56.3
  • a Mutations were introduced into a wt, day 0 env clone (pcDNA3.1-env) derived from patient 30-1 by using the Quik-Change kit (Stratagene Inc.) and degenerate oligonucleotide primers. Mutations were identified by nucleotide sequence analysis.