TABLE 3.

Distribution of MICs of PGE 9262932, PGE 9509924, and comparator fluoroquinolones for 69 isolates of S. pneumoniae with no mutations (wild type) in the four QRDRs or with a single mutation or combinations of mutations within the QRDRs of gyrA and parC

Amino acid alterationsNo. of isolatesQuinoloneNo. of isolates for which the MIC (μg/ml) was:
GyrAParC≤0.0080.0150.030.060.120.250.5124816≥32
NoneNone8aPGE 9262932332
PGE 9509924422
Moxifloxacin1322
Gatifloxacin152
Levofloxacin152
Ciprofloxacin1421
NoneNone21bPGE 92629321155
PGE 9509924111531
Moxifloxacin1146
Gatifloxacin12144
Levofloxacin15123
Ciprofloxacin15105
NoneLys137Asn6cPGE 9262932312
PGE 95099241221
Moxifloxacin6
Gatifloxacin6
Levofloxacin42
Ciprofloxacin132
NoneSer79Phe2dPGE 926293211
PGE 950992411
Moxifloxacin2
Gatifloxacin11
Levofloxacin11
Ciprofloxacin11
Ser81TyrNone3ePGE 92629323
PGE 950992421
Moxifloxacin12
Gatifloxacin111
Levofloxacin3
Ciprofloxacin111
Ser81PheNone2dPGE 926293211
PGE 950992411
Moxifloxacin11
Gatifloxacin11
Levofloxacin11
Ciprofloxacin11
Ser81PheSer79Phe16fPGE 92629322761
PGE 95099241465
Moxifloxacin691
Gatifloxacin484
Levofloxacin844
Ciprofloxacin466
Ser81PheSer79Phe, Lys137Asn4aPGE 926293222
PGE 950992422
Moxifloxacin31
Gatifloxacin22
Levofloxacin22
Ciprofloxacin22
Ser81PheAsp83Asn, Lys137Asn2aPGE 926293211
PGE 950992411
Moxifloxacin11
Gatifloxacin2
Levofloxacin2
Ciprofloxacin2
  • a All isolates had the wild-type sequences GyrB and ParE.

  • b Twelve isolates had the wild-type sequence at GyrB and the Ile460Val alteration in ParE, one isolate had the Arg541Lys alteration in GyrB and the Ile460Val alteration in ParE, one isolate had the Ala639Gln alteration in GyrB and the Ile460Val alteration in ParE, five isolates had the wild-type sequence at GyrB and the Lys466Met alteration in ParE, one isolate had the Ala639Gln alteration in GyrB and the wild-type sequence at ParE, and one isolate had the Ala538Ser alteration in GyrB and the wild-type sequence at ParE.

  • c Three isolates had wild-type sequences at GyrB and ParE, two isolates had the wild-type sequence at GyrB and the Ile460Val alteration in ParE, and the isolate had the Ala639Gln alteration in GyrB and the wild-type sequence at ParE.

  • d One isolate had wild-type sequences at GyrB and ParE, and one isolate had the wild-type sequence at GyrB and the Ile460Val alteration in ParE.

  • e Two isolates had wild-type sequences at GyrB and ParE, and one isolate had the wild-type sequence at GyrB and the Ile460Val alteration in ParE.

  • f Seven isolates had wild-type sequences at GyrB and ParE, five isolates had the wild-type sequences at GyrB and the Ile460Val alteration in ParE, one isolate had the wild-type sequence at GyrB and the Pro454Ser alteration in at ParE, one isolate had the Ala639Gln alteration in GyrB and the wild-type sequence at ParE, one isolate had the Arg541Lys alteration in GyrB and the Ile460Val alteration in ParE, and one isolate had the Ala639Gln alteration in GyrB and the Ile460Val alteration in ParE.