TABLE 1.

Identification, isolation, MIC, and resistance mechanism for isolate collection

Reference isolate no.Isolate designationStrain typeaHospital codeSite of isolationMIC (μg/ml)cGene(s) overexpressedERG11 sequence analysisd
FLCCLTITCKTC
195-142AAThroat >64 8 >8 4 CDR1, CDR2
295-68ABUnknown >64 4 >8 16 CDR1, CDR2
395-158AFOropharynx >64 4 8 4 Hot spot III
498-112BHBlood >64 2 >8 16 Complete gene
598-145eAGUrine >64 2 >8 16 Hot spot III
695-111eAFMouth >64 2 >8 4 CDR1, CDR2
795-16AFParacentesis fluid >64 2 >8 4 Complete gene
896-23AAThroat >64 2 1 8 Hot spot III
998-136CBPeritoneal dialysate >64 1 >8 16 Hot spot III
1095-188AJWrist tissue 64 2 >8 16
1195-165AFVaginal >64 4 >8 ≤0.5Complete gene
1295-157AITongue 64 4 2 ≤0.5
1395-190AKEsophageal tissue32 2 4 8 CDR1, CDR2
1495-120AABlood322 1 8
1595-133ABMouth 64 1 ≤0.5≤0.5 CDR1, CDR2
1698-24CLUrine80.5 1 1
1797-363ALAbdominal fluid2 2 ≤0.5 16
1898-144AGBlood20.5 >8 4
1997-224AFBlood20.5 2 1
2098-155AGBlood≤0.50.5 1 2
2198-143AAUrine≤0.5≤.25 >8 4
2296-25fCAAbdominal fluid320.5 1 ≤0.5 ERG11, MDR1 Hot spot III
2398-141CBIndwelling catheter8 2 ≤0.5≤0.5
2498-7AABlood4≤.25 >8 ≤0.5
2597-119fACAbdomen1 1 ≤0.5≤0.5
2698-216AAUrine/catheter1≤.25 >8 ≤0.5
2798-85fAAPeritoneal fluid≤0.50.5 1 ≤0.5CDR1, (CDR2)
2898-125AABlood≤0.5≤.25 2 ≤0.5CDR2
2997-87BABlood≤0.5≤.25≤0.5 8
3098-13AEParacentesis fluid≤0.5≤.25≤0.5 1
3195-175AAEndotracheal aspirate32≤.25≤0.5≤0.5
3297-197fABBlood20.5≤0.5≤0.5
3398-8ABOral≤0.50.5≤0.5≤0.5ERG11
3498-113ADPeritoneal fluid≤0.5≤.25≤0.5≤0.5
3598-126AAChest tube≤0.5≤.25≤0.5≤0.5
3698-146BAPleural tissue≤0.5≤.25≤0.5≤0.5
C1g2-76AOral0.25NAh≤0.5≤0.5
C17g12-99AOral≥64NA≤0.52 ERG11, CDR1, CDR2, MDR1
  • a Strain type, as determined by PCR and restriction enzyme digestion (19).

  • b Isolates were obtained from 12 hospitals throughout California, coded by letter.

  • c FLC, fluconazole; CLT, clotrimazole; ITC, itraconazole; KTC, ketoconazole. Results that indicate that the isolate was resistant according to the breakpoints discussed in the text are underlined.

  • d The ERG11 genes of some of the isolates were sequenced. Either the entire gene or hot spot III, which includes amino acids 405 to 488, was sequenced.

  • e Colonies were dark green on ChromAgar but were confirmed to be C. albicans.

  • f The isolates were originally parts of mixed infections.

  • g C1 and C17, control isolates 1 and 17, respectively, from a well-characterized matched set.

  • h NA, not available.