TABLE 1.

Comparison of sensitivities of JC53BL cells and PBMC to HIV-1 infection

ELISA result for and phenotype of primary HIV-1 isolateaPBMCbJC53BLc
Namep24 (pg/ml)PhenotypeTCID50p24:TCID50TCID50p24:TCID50
    K19.5 × 105NSI1.8 × 1055.32.3 × 1054.1
    K23.2 × 105SI4.6 × 1046.91.2 × 1052.7
    K34.2 × 105SI1.8 × 1052.34.5 × 1050.9
    K45.5 × 105NSI4.6 × 10411.99.5 × 1050.58
    K56.7 × 105SI4.6 × 10414.61.8 × 1053.7
    K68.2 × 105SI4.6 × 10417.88.5 × 1049.6
    K71.3 × 105SI1.2 × 10410.84.5 × 1042.9
    K86.8 × 105NSI4.6 × 10414.81.1 × 1056.2
    K94.2 × 105NSI4.6 × 1049.11.3 × 1053.2
    K105.3 × 105SI4.6 × 10411.51.1 × 1054.8
    K111.2 × 105SI1.8 × 1050.74.1 × 1050.29
    K125.4 × 105NSI4.6 × 10411.79.0 × 1046.0
    K136.5 × 104SI1.2 × 1045.43.8 × 1041.7
    K145.0 × 105NSI4.6 × 10410.92.1 × 1052.4
    K152.1 × 105NSI4.6 × 1044.61.2 × 1051.8
    K162.2 × 105NSI4.6 × 1044.83.6 × 1050.61
    K172.7 × 105SI1.2 × 10422.51.0 × 1052.7
    K182.6 × 105NSI4.6 × 1045.72.0 × 1051.3
    K195.1 × 105NSI4.6 × 10411.12.8 × 1051.8
    K202.1 × 105SI1.2 × 10417.54.5 × 1044.7
  • a Primary virus isolates were derived by coculture (10 days) of HIV-1-infected patient PBMC with PHA-stimulated normal donor PBMC. Virus concentrations were determined by HIV-1 p24 antigen enzyme-linked immunosorbent assay (ELISA) (Beckman-Coulter), and the syncytium-inducting (SI) or non-syncytium-inducing (NSI) phenotypes were determined on MT2 cells.

  • b Analysis of PBMC-derived HIV-1 infectivity by endpoint dilution culture of PHA-stimulated normal-donor PBMC. Virus titers for 50% tissue culture infectivity doses per milliliter (TCID50/ml) were calculated using the Spearman-Karber formula.

  • c Analysis of PBMC-derived HIV-1 infectivity by counting β-Gal positive JC53BL positive cell colonies under a microscope.