TABLE 3.

Ciprofloxacin inhibition of gyrase DNA supercoiling activity and stimulation of cleavage complex formation with gyrase

Recombinant enzymeIC50 (μg/ml) with the following K+-glutamate concn:CC50 (μg/ml) with the following K+-glutamate concn:
0 mM100 mM300 mM0 mM100 mM300 mM
E. coli GyrA2 GyrB20.16-0.310.62-1.250.62-1.250.310.16-0.310.62
Chimera 7 GyrA2 and E. coli GyrB20.62-1.250.16-0.310.62-1.250.62-1.251.255