TABLE 1.

Features of uropathogenic Escherichia coli wild-type strains and their derivativesa,c

StrainPAI-related genesMIC of Cip for wt strainExpt no. (loss rate), without CipExpt no. (passage/loss rate), with CipbMIC of Cip for derivativesVFs lost
HC14366 hly, cnf1, papA, -C, -EF, -G, fyu, sat1, hra 0.0121 (<10−5)1 (1/1 × 10−3)0.12 hly, cnf1, sat1
2 (<10−5)2 (2/2 × 10−3)0.032 hly, cnf1, sat1
3 (<10−5)3 (4/5 × 10−3)0.016 hly, cnf1, sat1
HC109 hly, cnf1, papA, -C, -EF, -G, sfa, iroN, fyu, hra 0.0081 (<10−5)1 (4/1 × 10−3)0.016 hly, cnf1, hra
2 (<10−5)2 (1/7 × 10−4) 2 (1/4.8 × 10−4)0.032 hly, cnf1, hra
3 (<10−5)3 (1/2.6 × 10−4)0.032 hly, cnf1, hra
HC359 hly, cnf1, papA, -C, -EF, -G, sfa, iroN, fyu, hra 0.0061 (<10−5)1 (4/1 × 10−4)*0.19 hly, cnf1, hra
2 (<10−5)2 (2/4 × 10−4)*0.06 hly, cnf1, hra
3 (<10−5)3 (4/2.8 × 10−4)*0.19 hly, cnf1, hra
HC14366-recA mutant hly, cnf1, papA, -C, -EF, -G, fyu, sat1, hra 0.0121 (<10−5)1 (<10−5)0.016
2 (<10−5)2 (<10−5)0.016
3 (<10−5)3 (<10−5)0.016
HC109-recA mutant hly, cnf1, papA, -C, -EF, -G, sfa, iroN, fyu, hra 0.0081 (<10−5)1 (2/5 × 10−4)0.064 hly, cnf1, hra
2 (<10−5)2 (2/1 × 10−4)0.032 hly, cnf1, hra
3 (<10−5)3 (2/3 × 10−4)0.032 hly, cnf1, hra
  • a Cip, ciprofloxacin; wt, wild type; VFs, virulence factors.

  • b *, isolates showing a mutation in the Ser-83 codon of the gyrA gene.

  • c MICs are in mg/liter.