TABLE 2.

Sequences of oligonucleotides used for PCR mapping

PCRaPrimerTargetPrimer sequence
Horf341 5′Between CR1 and p(Kp760-2ind)5′-TGCATGGAAAAGCGTGGGAT-3′
rot 3′5′-AACATCACCCGCTCGCTGG-3′
IsapB 5′Between sap operon and psp operon5′-GCCAGCTAAAGACCATCTCG-3′
pspF 3′5′-CCAGCGATTACCCACTGAAT-3′
JpspF 5′Inside psp operon5′-CAGATCCAGCGGTAGGGTTA-3′
pspA 3′5′-CAACATCAATGCCTGCTGAC-3′
KFaz 5′Between psp operon and M. morganii region5′-AAGAAACAGTGTGCCGCCAT-3′
morg 3′5′-GGTAACGCTGATCGGTAAAC-3′
LpspD 5′Between psp operon and M. morganii region5′-CCGGCAATCAGGCTAAATAA-3′
ampC 3′5′-TCCGGAAAAACAGGTGGCGA-3′
MDHA-1 upperM. morganii region5′-CTCATCCTCCATAAAACAGC-3′
DHA-1 lower5′-TTATCTCACACCTTTATTACT-3′
NDHA-2 upperM. morganii region5′-AGATACATTGCCATTTCCAG-3′
DHA-2 lower5′-ACTTGCCGCCGTTACTCACA-3′
ODHA-3 upperM. morganii region5′-TGTGCCATCAGCGGTTTATT-3′
DHA-3 lower5′-TGGAAGGTGAGTGAGTTTTA-3′
PampR 5′Second copy of 3′-CS5′-CGTGAGCGGGTGAAAGTCG-3′
orf5 3′5′-AAGTGTCGACGTGGGTGAAT-3′
Qsul5′Downstream from second copy of 3′-CS5′-ATCAGATGCACCGTGTTTCA-3′
IS6100 3′5′-GGTGATCGCTGCACCATAG-3′
Rorf5 5′Downstream from second copy of 3′-CS5′-CCGCATATCTGCACAAGCTC-3′
tniB 3′5′-AGTTGGAAAATCGCTTCGAG-3′
Sorf5 5′Downstream from second copy of 3′-CS5′-CCGCATATCTGCACAAGCTC-3′
IS26 3′5′-CCCAGGGGATCACCATAATA-3′
Tamont KpBUpstream from p(TN60013-2ind)5′-CTTATCATCCCCTTTTGCTG-3′
pspF 3′5′-CCAGCGATTACCCACTGAAT-3′
Umorg 5′M. morganii chromosome5′-GCGGGCTCATAAAGATTTCA-3′
morg 3′5′-TGGCGTTAGGACTGGAAAAC-3′
iGSP1iDownstream from CR15′-TACCGCCAAATCGAACCTTATTAGAGC-3′
*GSP2 ib5′-TTTAGGCTGGACCGGCAGTTAAAAT-3′
iiGSP1iiUpstream from ampC5′-ATAACCGTGCTTCCTGGGTGCATAAAAC-3′
*GSP2 iib5′-AAAAACAGGTGGCGATTGTGATTCTGG-3′
  • a i and ii, PCR with the Universal Genome Walker kit.

  • b Primer for nested PCR.